Prodrugs of mitotic kinesin inhibitors

ABSTRACT

The present invention relates to prodrugs of dihydropyrazole compounds that are useful for treating cellular proliferative diseases, for treating disorders associated with KSP kinesin activity, and for inhibiting KSP kinesin. The invention also related to compositions which comprise these compounds, and methods of using them to treat cancer in mammals.

PRIORITY CLAIM

This application is a 371 application of PCT/US2005/022879 that wasfiled on Jun. 27, 2005, which claims priority from the U.S. ProvisionalApplication Nos. 60/584,517, filed on Jul. 1, 2004, and 60/605,840,filed on Aug. 30, 2004, now expired.

BACKGROUND OF THE INVENTION

This invention relates to prodrug derivatives of dihydropyrazolecompounds that are inhibitors of mitotic kinesins, in particular themitotic kinesin KSP, and are useful in the treatment of cellularproliferative diseases, for example cancer, hyperplasias, restenosis,cardiac hypertrophy, immune disorders and inflammation.

Among the therapeutic agents used to treat cancer are the taxanes andvinca alkaloids. Taxanes and vinca alkaloids act on microtubules, whichare present in a variety of cellular structures. Microtubules are theprimary structural element of the mitotic spindle. The mitotic spindleis responsible for distribution of replicate copies of the genome toeach of the two daughter cells that result from cell division. It ispresumed that disruption of the mitotic spindle by these drugs resultsin inhibition of cancer cell division, and induction of cancer celldeath. However, microtubules form other types of cellular structures,including tracks for intracellular transport in nerve processes. Becausethese agents do not specifically target mitotic spindles, they have sideeffects that limit their usefulness.

Improvements in the specificity of agents used to treat cancer is ofconsiderable interest because of the therapeutic benefits which would berealized if the side effects associated with the administration of theseagents could be reduced. Traditionally, dramatic improvements in thetreatment of cancer are associated with identification of therapeuticagents acting through novel mechanisms. Examples of this include notonly the taxanes, but also the camptothecin class of topoisomerase Iinhibitors. From both of these perspectives, mitotic kinesins areattractive targets for new anti-cancer agents.

Mitotic kinesins are enzymes essential for assembly and function of themitotic spindle, but are not generally part of other microtubulestructures, such as in nerve processes. Mitotic kinesins play essentialroles during all phases of mitosis. These enzymes are “molecular motors”that transform energy released by hydrolysis of ATP into mechanicalforce which drives the directional movement of cellular cargoes alongmicrotubules. The catalytic domain sufficient for this task is a compactstructure of approximately 340 amino acids. During mitosis, kinesinsorganize microtubules into the bipolar structure that is the mitoticspindle. Kinesins mediate movement of chromosomes along spindlemicrotubules, as well as structural changes in the mitotic spindleassociated with specific phases of mitosis. Experimental perturbation ofmitotic kinesin function causes malformation or dysfunction of themitotic spindle, frequently resulting in cell cycle arrest and celldeath.

Among the mitotic kinesins which have been identified is KSP. KSPbelongs to an evolutionarily conserved kinesin subfamily of plusend-directed microtubule motors that assemble into bipolar homotetramersconsisting of antiparallel homodimers. During mitosis KSP associateswith microtubules of the mitotic spindle. Microinjection of antibodiesdirected against KSP into human cells prevents spindle pole separationduring prometaphase, giving rise to monopolar spindles and causingmitotic arrest and induction of programmed cell death. KSP and relatedkinesins in other, non-human, organisms, bundle antiparallelmicrotubules and slide them relative to one another, thus forcing thetwo spindle poles apart. KSP may also mediate in anaphase B spindleelongation and focussing of microtubules at the spindle pole.

Human KSP (also termed HsEg5) has been described [Blangy, et al., Cell,83:1159-69 (1995); Whitehead, et al., Arthritis Rheum., 39:1635-42(1996); Galgio et al., J. Cell Biol., 135:339-414 (1996); Blangy, etal., J Biol. Chem., 272:19418-24 (1997); Blangy, et al., Cell MotilCytoskeleton, 40:174-82 (1998); Whitehead and Rattner, J. Cell Sci.,111:2551-61 (1998); Kaiser, et al., JBC 274:18925-31 (1999); GenBankaccession numbers: X85137, NM004523 and U37426], and a fragment of theKSP gene (TRIP5) has been described [Lee, et al., Mol Endocrinol.,9:243-54 (1995); GenBank accession number L40372]. Xenopus KSP homologs(Eg5), as well as Drosophila K-LP61 F/KRP 130 have been reported.

Certain quinazolinones have recently been described as being inhibitorsof KSP (PCT Publ. WO 01/30768, May 3, 2001).

Mitotic kinesins are attractive targets for the discovery anddevelopment of novel mitotic chemotherapeutics. Accordingly, it is anobject of the present invention to provide compounds, methods andcompositions useful in the inhibition of KSP, a mitotic kinesin.

SUMMARY OF THE INVENTION

The present invention relates to prodrugs of dihydropyrazolederivatives, that are useful for treating cellular proliferativediseases, for treating disorders associated with KSP kinesin activity,and for inhibiting KSP kinesin. The compounds of the invention may beillustrated by the Formula I:

DETAILED DESCRIPTION OF THE INVENTION

The compounds of this invention are useful in the inhibition of mitotickinesins and are illustrated by a compound of Formula I:

or a pharmaceutically acceptable salt or stereoisomer thereof, whereina is independently 0 or 1;b is independently 0 or 1;m is independently 0, 1, or 2;n is 0 to 8;p is 1 to 6;u is 1, 2, 3, 4 or 5;R¹ is selected from:

1) (C═O)C₁-C₁₀ alkyl,

2) (C═O)aryl,

3) (C═O)C₂-C₁₀ alkenyl,

4) (C═O)C₂-C₁₀ alkynyl,

5) (C═O)C₃-C₈ cycloalkyl,

6) (C═O)heterocyclyl,

7) (C═O)NR⁹R¹⁰,

8) (C═O)OC₁-C₁₀ alkyl,

9) SO₂NR⁹R¹⁰,

10) SO₂C₁-C₁₀ alkyl,

11) SO₂C₁-C₁₀ aryl,

12) SO₂C₁-C₁₀ heterocyclyl,

13) C₁-C₁₀ alkyl,

14) aryl,

15) heteroaryl,

16) (CH₂)_(u)(C═O)C₁-C₁₀ alkyl,

17) (CH₂)_(u)(C═O)NR⁹R¹⁰,

18) 3-pyrrolidinonyl, 3-piperidinonyl, 2-cyclopentanonyl,2-cyclohexanonyl,

19) (C═O)(C═O)C₁-C₁₀ alkyl,

20) (C═O)(C═O)NR⁹R¹⁰,

21) (C═O)(C═O)OC₁-C₁₀ alkyl,

said alkyl, aryl, alkenyl, alkynyl, cycloalkyl, heteroaryl andheterocyclyl is optionally substituted with one or more substituentsselected from R⁷; or

R² is selected from:

1) C₁-C₁₀ alkyl,

2) aryl,

3) C₂-C₁₀ alkenyl,

4) C₂-C₁₀ alkynyl,

5) C₁-C₆ perfluoroalkyl,

6) C₁-C₆ aralkyl,

7) C₁-C₆ heteroaralkyl,

8) C₃-C₈ cycloalkyl, and

9) heterocyclyl,

said alkyl, aryl, alkenyl, alkynyl, cycloalkyl, aralkyl, heteroaralkyland heterocyclyl is optionally substituted with one or more substituentsselected from R⁷;

R³, R⁴ and R⁵ are independently selected from:

1) H,

2) C₁-C₁₀ alkyl,

3) aryl,

4) C₂-C₁₀ alkenyl,

5) C₂-C₁₀ alkynyl,

6) C₁-C₆ perfluoroalkyl,

7) C₁-C₆ aralkyl,

8) C₃-C₈ cycloalkyl, and

9) heterocyclyl,

said alkyl, aryl, alkenyl, alkynyl, cycloalkyl, aralkyl and heterocyclylis optionally substituted with one or more substituents selected fromR⁷; or

R³ and R⁴ attached to the same carbon atom are combined to form—(CH₂)_(u)— wherein one of the carbon atoms is optionally replaced by amoiety selected from O, S(O)_(m), —N(R⁹)C(O)—, and —N(COR¹⁰)—;

R⁷ is:

1) (C═O)_(a)O_(b)C₁-C₁₀ alkyl,

2) (C═O)_(a)O_(b)aryl,

3) C₂-C₁₀ alkenyl,

4) C₂-C₁₀ alkynyl,

5) (C═O)_(a)O_(b) heterocyclyl,

6) CO₂H,

7) halo,

8) CN,

9) OH,

10) O_(b)C₁-C₆ perfluoroalkyl,

11) O_(a)(C═O)_(b)NR⁹R¹⁰,

12) S(O)_(m)R^(a),

13) S(O)₂NR⁹R¹⁰,

14) oxo,

15) CHO,

16) (N═O)R⁹R¹⁰, or

17) (C═O)_(a)O_(b)C₃-C₈ cycloalkyl,

said alkyl, aryl, alkenyl, alkynyl, heterocyclyl, and cycloalkyloptionally substituted with one or more substituents selected from R⁸;

R⁸ is selected from:

1) (C═O)_(a)O_(b)(C₁-C₁₀)alkyl,

2) O_(b)(C₁-C₃)perfluoroalkyl,

3) (C₀-C₆)alkylene-S(O)_(m)R^(a),

4) oxo,

5) OH,

6) halo,

7) CN,

8) (C═O)_(a)O_(b)(C₂-C₁₀)alkenyl,

9) (C═O)_(a)O_(b)(C₂-C₁₀)alkynyl,

10) (C═O)_(a)O_(b)(C₃-C₆)cycloalkyl,

11) (C═O)_(a)O_(b)(C₀-C₆)alkylene-aryl,

12) (C═O)_(a)O_(b)(C₀-C₆)alkylene-heterocyclyl,

13) (C═O)_(a)O_(b)(C₀-C₆)alkylene-N(R^(b))₂,

14) C(O)R^(a),

15) (C₀-C₆)alkylene-CO₂R^(a),

16) C(O)H,

17) (C₀-C₆)alkylene-CO₂H,

18) C(O)N(R^(b))₂,

19) S(O)_(m)R^(a),

20) S(O)₂NR⁹R¹⁰, and

21) C(NH)NH₂;

said alkyl, alkenyl, alkynyl, cycloalkyl, aryl, and heterocyclyl isoptionally substituted with up to three substituents selected fromR^(b), OH, (C₁-C₆)alkoxy, halogen, CO₂H, CN, O(C═O)C₁-C₆ alkyl, oxo, andN(R^(b))₂;

R⁹ and R¹⁰ are independently selected from:

1) H,

2) (C═O)O_(b)C₁-C₁₀ alkyl,

3) (C═O)O_(b)C₃-C₈ cycloalkyl,

4) (C═O)O_(b)aryl,

5) (C═O)O_(b)heterocyclyl,

6) C₁-C₁₀ alkyl,

7) aryl,

8) C₂-C₁₀ alkenyl,

9) C₂-C₁₀ alkynyl,

10) heterocyclyl,

11) C₃-C₈ cycloalkyl,

12) SO₂R^(a), and

13) (C═O)NR^(b) ₂,

said alkyl, cycloalkyl, aryl, heterocylyl, alkenyl, and alkynyl isoptionally substituted with one or more substituents selected from R⁸,or

R⁹ and R¹⁰ can be taken together with the nitrogen to which they areattached to form a monocyclic or bicyclic heterocycle with 3-7 membersin each ring and optionally containing, in addition to the nitrogen, oneor two additional heteroatoms selected from N, O and S, said monocyclicor bicyclic heterocycle optionally substituted with one or moresubstituents selected from R⁸;

-   R^(a) is (C₁-C₆)alkyl, (C₃-C₆)cycloalkyl, aryl, benzyl or    heterocyclyl;-   R^(b) is H, (C₁-C₆)alkyl, aryl, heterocyclyl, (C₃-C₆)cycloalkyl,    (C═O)OC₁-C₆ alkyl, (C═O)C₁-C₆ alkyl or S(O)₂R^(a); and-   R^(c) is independently selected from: (C₁-C₆)alkoxy,    pivaloyloxymethoxy and NR^(b) ₂, or    two R^(c)s can be taken together with the phosphorous to which they    are attached to form a monocyclic heterocycle with 5-7 members said    monocyclic heterocycle optionally substituted with one, two or three    substituents selected from R^(b);    R^(d) is independently selected from H, (C₁-C₆)alkyl,    (C₃-C₆)cycloalkyl, aryl, benzyl or heterocyclyl;-   RP is selected from: —PO(OH)₂, —PO(R^(c))₂, —C(O)CH₂CH₂CH₂OPO(OH)₂,    C(O)CH₂CH₂CH₂OPO(R^(c))₂, —CH₂OPO(OH)₂, —CH₂OPO(R^(c))₂,    —C(O)(CHR^(d))_(p)NR^(a) ₂, —C(O)(CHR^(d))_(p)NR^(a) ₃ ⁺,    —CH₂OC(O)(CHR^(d))_(p)NR^(a) ₂, —CH₂OC(O)(CHR^(a))_(p)NR^(a) ₃ ⁺,    and

A further embodiment of the present invention is illustrated by acompound of Formula II:

or a pharmaceutically acceptable salt or stereoisomer thereof,wherein:a is independently 0 or 1;b is independently 0 or 1;m is independently 0, 1, or 2;n is 0 to 5;R¹ is selected from:

1) (C═O)C₁-C₁₀ alkyl,

2) (C═O)aryl,

3) (C═O)C₂-C₁₀ alkenyl,

4) (C═O)C₂-C₁₀ alkynyl,

5) (C═O)C₃-C₈ cycloalkyl,

6) (C═O)heterocyclyl,

7) (C═O)OC₁-C₁₀ alkyl,

8) (C═O)NR⁹R¹⁰,

9) SO₂NR⁹R¹⁰,

10) SO₂C₁-C₁₀ alkyl,

11) SO₂C₁-C₁₀ aryl,

12) SO₂C₁-C₁₀ heterocyclyl,

said alkyl, aryl, alkenyl, alkynyl, cycloalkyl, and heterocyclyl isoptionally substituted with one or more substituents selected from R⁷;or

R² is selected from:

1) C₁-C₁₀ alkyl,

2) aryl,

3) C₂-C₁₀ alkenyl,

4) C₂-C₁₀ alkynyl,

5) C₁-C₆ perfluoroalkyl,

6) C₁-C₆ aralkyl,

7) C₃-C₈ cycloalkyl, and

8) heterocyclyl,

said alkyl, aryl, alkenyl, alkynyl, cycloalkyl, aralkyl and heterocyclylis optionally substituted with one or more substituents selected fromR⁷;

R³, R⁴ and R⁵ are independently selected from:

1) H,

2) C₁-C₁₀ alkyl,

3) aryl,

4) C₂-C₁₀ alkenyl,

5) C₂-C₁₀ alkynyl,

6) C₁-C₆ perfluoroalkyl,

7) C₁-C₆ aralkyl,

8) C₃-C₈ cycloalkyl, and

9) heterocyclyl,

said alkyl, aryl, alkenyl, alkynyl, cycloalkyl, aralkyl and heterocyclylis optionally substituted with one or more substituents selected fromR⁷;

R⁷ is:

1) (C═O)_(a)O_(b)C₁-C₁₀ alkyl,

2) (C═O)_(a)O_(b)aryl,

3) C₂-C₁₀ alkenyl,

4) C₂-C₁₀ alkynyl,

5) (C═O)_(a)O_(b) heterocyclyl,

6) CO₂H,

7) halo,

8) CN,

9) OH,

10) O_(b)C₁-C₆ perfluoroalkyl,

11) O_(a)(C═O)_(b)NR⁹R¹⁰,

12) S(O)_(m)R^(a),

13) S(O)₂NR⁹R¹⁰,

14) oxo,

15) CHO,

16) (N═O)R⁹R¹⁰, or

17) (C═O)_(a)O_(b)C₃-C₈ cycloalkyl,

said alkyl, aryl, alkenyl, alkynyl, heterocyclyl, and cycloalkyloptionally substituted with one, two or three substituents selected fromR⁸;

R⁸ is selected from:

1) (C═O)_(a)O_(b)(C₁-C₁₀)alkyl,

2) O_(b)(C₁-C₃)perfluoroalkyl,

3) oxo,

4) OH,

5) halo,

6) CN,

7) (C₂-C₁₀)alkenyl,

8) (C₂-C₁₀)alkynyl,

9) (C═O)_(a)O_(b)(C₃-C₆)cycloalkyl,

10) (C═O)_(a)O_(b)(C₀-C₆)alkylene-aryl,

11) (C═O)_(a)O_(b)(C₀-C₆)alkylene-heterocyclyl,

12) (C═O)_(a)O_(b)(C₀-C₆)alkylene-N(R^(b))₂,

13) C(O)R^(a),

14) (C₀-C₆)alkylene-CO₂R^(a),

15) C(O)H,

16) (C₀-C₆)alkylene-CO₂H, and

17) C(O)N(R^(b))₂,

18) S(O)_(m)R^(a), and

19) S(O)₂NR⁹R¹⁰

said alkyl, alkenyl, alkynyl, cycloalkyl, aryl, and heterocyclyl isoptionally substituted with up to three substituents selected fromR^(b), OH, (C₁-C₆)alkoxy, halogen, CO₂H, CN, O(C═O)C₁-C₆ alkyl, oxo, andN(R^(b))₂;

R⁹ and R¹⁰ are independently selected from:

1) H,

2) (C═O)O_(b)C₁-C₁₀ alkyl,

3) (C═O)O_(b)C₃-C₈ cycloalkyl,

4) (C═O)O_(b)aryl,

5) (C═O)O_(b)heterocyclyl,

6) C₁-C₁₀ alkyl,

7) aryl,

8) C₂-C₁₀ alkenyl,

9) C₂-C₁₀ alkynyl,

10) heterocyclyl,

11) C₃-C₈ cycloalkyl,

12) SO₂R^(a), and

13) (C═O)NR^(b) ₂,

said alkyl, cycloalkyl, aryl, heterocylyl, alkenyl, and alkynyl isoptionally substituted with one, two or three substituents selected fromR⁸, or

R⁹ and R¹⁰ can be taken together with the nitrogen to which they areattached to form a monocyclic or bicyclic heterocycle with 5-7 membersin each ring and optionally containing, in addition to the nitrogen, oneor two additional heteroatoms selected from N, O and S, said monocyclicor bicyclic heterocycle optionally substituted with one, two or threesubstituents selected from R⁸;

-   R^(a) is (C₁-C₆)alkyl, (C₃-C₆)cycloalkyl, aryl, or heterocyclyl;-   R^(b) is H, (C₁-C₆)alkyl, aryl, heterocyclyl, (C₃-C₆)cycloalkyl,    (C═O)OC₁-C₆ alkyl, (C═O)C₁-C₆ alkyl or S(O)₂R^(a); and-   R^(c) is independently selected from: (C₁-C₆)alkoxy,    pivaloyloxymethoxy and NR^(b) ₂, or    two R^(c)s can be taken together with the phosphorous to which they    are attached to form a monocyclic heterocycle with 5-7 members said    monocyclic heterocycle optionally substituted with one, two or three    substituents selected from R^(b);-   RP is selected from: —PO(OH)₂, —PO(R^(c))₂, —C(O)CH₂CH₂CH₂OPO(OH)₂,    C(O)CH₂CH₂CH₂OPO(R^(c))₂, —C(O)CH₂NR^(a) ₂, —C(O)CH₂NR^(a) ₃ ⁺,    —CH₂OC(O)CH₂NR^(a) ₂, —CH₂OC(O)CH₂NR^(a) ₃ ⁺, and

Another embodiment is the compound of the Formula II describedimmediately above, or a pharmaceutically acceptable salt or stereoisomerthereof, wherein:

R¹ is selected from:

1) (C═O)C₁-C₁₀ alkyl,

2) (C═O)aryl,

3) (C═O)C₃-C₈ cycloalkyl,

4) (C═O)heterocyclyl,

5) (C═O)OC₁-C₁₀ alkyl,

6) SO₂NR⁹R¹⁰, and

7) SO₂C₁-C₁₀ alkyl,

said alkyl, aryl, cycloalkyl, and heterocyclyl is optionally substitutedwith one, two or three substituents selected from R⁷;

R² is selected from:

1) C₁-C₁₀ alkyl,

2) aryl, and

3) heteroaryl,

said alkyl, aryl and heteroaryl is optionally substituted with one ormore substituents selected from R⁷;

R³ and R⁴ are independently selected from:

1) H, and

2) C₁-C₁₀ alkyl,

said alkyl is optionally substituted with one or more substituentsselected from R⁷; and

R⁵ is selected from:

1) H,

2) C₁-C₁₀ alkyl,

3) aryl, and

4) heterocyclyl,

said alkyl, aryl and heterocyclyl is optionally substituted with one ormore substituents selected from R⁷;

-   RP is selected from: —PO(OH)₂, —C(O)CH₂CH₂CH₂OPO(OH)₂, —CH₂OPO(OH)₂,    —C(O)CH₂NR^(a) ₂, —C(O)CH₂NR^(a) ₃ ⁺, —CH₂OC(O)CH₂NR^(a) ₂,    —CH₂OC(O)CH₂NR^(a) ₃ ⁺, and

R⁷, R⁸, R⁹, R¹⁰, R^(a) and R^(b) are as described immediately above.

Another embodiment of the present invention is illustrated by a compoundof Formula III:

or a pharmaceutically acceptable salt or stereoisomer thereof, whereina is independently 0 or 1;b is independently 0 or 1;m is independently 0, 1, or 2;n is 0 to 5;R¹ is selected from:

1) (C═O)C₁-C₁₀ alkyl,

2) (C═O)aryl,

3) (C═O)C₂-C₁₀ alkenyl,

4) (C═O)C₂-C₁₀ alkynyl,

5) (C═O)C₃-C₈ cycloalkyl,

6) (C═O)heterocyclyl,

7) (C═O)OC₁-C₁₀ alkyl,

8) (C═O)NR⁹R¹⁰,

9) SO₂NR⁹R¹⁰,

10) SO₂C₁-C₁₀ alkyl,

11) SO₂C₁-C₁₀ aryl,

12) SO₂C₁-C₁₀ heterocyclyl,

said alkyl, aryl, alkenyl, alkynyl, cycloalkyl, and heterocyclyl isoptionally substituted with one or more substituents selected from R⁷;or

R^(7a) and R^(7c) are independently selected from:

1) H,

2) O_(b)C₁-C₁₀ alkyl,

3) halo,

4) CN, and

5) OH,

said alkyl optionally substituted with one, two or three substituentsselected from R⁸;

R^(7b) is halogen;

R⁷ is:

1) (C═O)_(a)O_(b)C₁-C₁₀ alkyl,

2) (C═O)_(a)O_(b)aryl,

3) C₂-C₁₀ alkenyl,

4) C₂-C₁₀ alkynyl,

5) (C═O)_(a)O_(b) heterocyclyl,

6) CO₂H,

7) halo,

8) CN,

9) OH,

10) O_(b)C₁-C₆ perfluoroalkyl,

11) O_(a)(C═O)_(b)NR⁹R¹⁰,

12) S(O)_(m)R^(a),

13) S(O)₂NR⁹R¹⁰,

14) oxo,

15) CHO,

16) (N═O)R⁹R¹⁰, or

17) (C═O)_(a)O_(b)C₃-C₈ cycloalkyl,

said alkyl, aryl, alkenyl, alkynyl, heterocyclyl, and cycloalkyloptionally substituted with one or more substituents selected from R⁸;

R⁸ is selected from:

1) (C═O)_(a)O_(b)(C₁-C₁₀)alkyl,

2) O_(b)(C₁-C₃)perfluoroalkyl,

3) oxo,

4) OH,

5) halo,

6) CN,

7) (C₂-C₁₀)alkenyl,

8) (C₂-C₁₀)alkynyl,

9) (C═O)_(a)O_(b)(C₃-C₆)cycloalkyl,

10) (C═O)_(a)O_(b)(C₀-C₆)alkylene-aryl,

11) (C═O)_(a)O_(b)(C₀-C₆)alkylene-heterocyclyl,

12) (C═O)_(a)O_(b)(C₀-C₆)alkylene-N(R^(b))₂,

13) C(O)R^(a),

14) (C₀-C₆)alkylene-CO₂R^(a),

15) C(O)H,

16) (C₀-C₆)alkylene-CO₂H, and

17) C(O)N(R^(b))₂,

18) S(O)_(m)R^(a), and

19) S(O)₂NR⁹R¹⁰;

said alkyl, alkenyl, alkynyl, cycloalkyl, aryl, and heterocyclyl isoptionally substituted with up to three substituents selected fromR^(b), OH, (C₁-C₆)alkoxy, halogen, CO₂H, CN, O(C═O)C₁-C₆ alkyl, oxo, andN(R^(b))₂;

R⁹ and R¹⁰ are independently selected from:

1) H,

2) (C═O)O_(b)C₁-C₁₀ alkyl,

3) (C═O)O_(b)C₃-C₈ cycloalkyl,

4) (C═O)O_(b)aryl,

5) (C═O)O_(b)heterocyclyl,

6) C₁-C₁₀ alkyl,

7) aryl,

8) C₂-C₁₀ alkenyl,

9) C₂-C₁₀ alkynyl,

10) heterocyclyl,

11) C₃-C₈ cycloalkyl,

12) SO₂R^(a), and

13) (C═O)NR^(b) ₂,

said alkyl, cycloalkyl, aryl, heterocylyl, alkenyl, and alkynyl isoptionally substituted with one, two or three substituents selected fromR⁸, or

R⁹ and R¹⁰ can be taken together with the nitrogen to which they areattached to form a monocyclic or bicyclic heterocycle with 5-7 membersin each ring and optionally containing, in addition to the nitrogen, oneor two additional heteroatoms selected from N, O and S, said monocyclicor bicyclic heterocycle optionally substituted with one, two or threesubstituents selected from R⁸;R^(a) is independently selected from: (C₁-C₆)alkyl, (C₃-C₆)cycloalkyl,aryl, and heterocyclyl;R^(b) is independently selected from: H, (C₁-C₆)alkyl, aryl,heterocyclyl, (C₃-C₆)cycloalkyl, (C═O)OC₁-C₆ alkyl, (C═O)C₁-C₆ alkyl orS(O)₂R^(a); and

-   RP is selected from: —PO(OH)₂, —C(O)CH₂CH₂CH₂OPO(OH)₂, —CH₂OPO(OH)₂,    —C(O)CH₂NR^(a) ₂, —C(O)CH₂NR^(a) ₃ ⁺, —CH₂OC(O)CH₂NR^(a) ₂,    —CH₂OC(O)CH₂NR^(a) ₃ ⁺, and

Another embodiment of the present invention is illustrated by a compoundof Formula IV:

or a pharmaceutically acceptable salt or stereoisomer thereof, whereina is independently 0 or 1;b is independently 0 or 1;m is independently 0, 1, or 2;n is 0 to 5;R^(7a) and R^(7c) are independently selected from:

1) O_(b)C₁-C₁₀ alkyl,

2) halo,

3) CN, and

4) OH,

said alkyl optionally substituted with one, two or three substituentsselected from R⁸;

R^(7b) is halogen;

R⁷ is:

1) (C═O)_(a)O_(b)C₁-C₁₀ alkyl,

2) (C═O)_(a)O_(b)aryl,

3) C₂-C₁₀ alkenyl,

4) C₂-C₁₀ alkynyl,

5) (C═O)_(a)O_(b) heterocyclyl,

6) CO₂H,

7) halo,

8) CN,

9) OH,

10) O_(b)C₁-C₆ perfluoroalkyl,

11) O_(a)(C═O)_(b)NR⁹R¹⁰,

12) S(O)_(m)R^(a),

13) S(O)₂NR⁹R¹⁰,

14) oxo,

15) CHO,

16) (N═O)R⁹R¹⁰, or

17) (C═O)_(a)O_(b)C₃-C₈ cycloalkyl,

said alkyl, aryl, alkenyl, alkynyl, heterocyclyl, and cycloalkyloptionally substituted with one or more substituents selected from R⁸;

R⁸ is selected from:

1) (C═O)_(a)O_(b)(C₁-C₁₀)alkyl,

2) O_(b)(C₁-C₃)perfluoroalkyl,

3) oxo,

4) OH,

5) halo,

6) CN,

7) (C₂-C₁₀)alkenyl,

8) (C₂-C₁₀)alkynyl,

9) (C═O)_(a)O_(b)(C₃-C₆)cycloalkyl,

10) (C═O)_(a)O_(b)(C₀-C₆)alkylene-aryl,

11) (C═O)_(a)O_(b)(C₀-C₆)alkylene-heterocyclyl,

12) (C═O)_(a)O_(b)(C₀-C₆)alkylene-N(R^(b))₂,

13) C(O)R^(a),

14) (C₀-C₆)alkylene-CO₂R^(a),

15) C(O)H,

16) (C₀-C₆)alkylene-CO₂H, and

17) C(O)N(R^(b))₂,

18) S(O)_(m)R^(a), and

19) S(O)₂NR⁹R¹⁰;

said alkyl, alkenyl, alkynyl, cycloalkyl, aryl, and heterocyclyl isoptionally substituted with up to three substituents selected fromR^(b), OH, (C₁-C₆)alkoxy, halogen, CO₂H, CN, O(C═O)C₁-C₆ alkyl, oxo, andN(R^(b))₂;

R⁹ and R¹⁰ are independently selected from:

1) H,

2) (C═O)O_(b)C₁-C₁₀ alkyl,

3) (C═O)O_(b)C₃-C₈ cycloalkyl,

4) (C═O)O_(b)aryl,

5) (C═O)O_(b)heterocyclyl,

6) C₁-C₁₀ alkyl,

7) aryl,

8) C₂-C₁₀ alkenyl,

9) C₂-C₁₀ alkynyl,

10) heterocyclyl,

11) C₃-C₈ cycloalkyl,

12) SO₂R^(a), and

13) (C═O)NR^(b) ₂,

said alkyl, cycloalkyl, aryl, heterocylyl, alkenyl, and alkynyl isoptionally substituted with one, two or three substituents selected fromR⁸, or

R⁹ and R¹⁰ can be taken together with the nitrogen to which they areattached to form a monocyclic or bicyclic heterocycle with 5-7 membersin each ring and optionally containing, in addition to the nitrogen, oneor two additional heteroatoms selected from N, O and S, said monocyclicor bicyclic heterocycle optionally substituted with one, two or threesubstituents selected from R⁸;R¹¹ is selected from:

1) C₁-C₁₀ alkyl,

2) aryl,

3) C₃-C₈ cycloalkyl,

4) heterocyclyl,

said alkyl, aryl, cycloalkyl, and heterocyclyl is optionally substitutedwith one or more substituents selected from R¹⁰; or

R^(a) is independently selected from: (C₁-C₆)alkyl, (C₃-C₆)cycloalkyl,aryl, and heterocyclyl; and

R^(b) is independently selected from: H, (C₁-C₆)alkyl, aryl,heterocyclyl, (C₃-C₆)cycloalkyl, (C═O)OC₁-C₆ alkyl, (C═O)C₁-C₆ alkyl orS(O)₂R^(a).

Specific examples of the compounds of the instant invention include:

-   3-[(5S)-1-acetyl-3-(2,5-difluorophenyl)-5-phenyl-4,5-dihydro-1H-pyrazol-5-yl]propan-1-ol;-   3-[(5S)-1-acetyl-3-(2,5-difluorophenyl)-5-phenyl-4,5-dihydro-1H-pyrazol-5-yl]propyl    dihydrogen phosphate;-   2-[(5R)-1-acetyl-3-(2-fluoro-5-methylphenyl)-5-phenyl-4,5-dihydro-1H-pyrazol-5-yl]ethanol;-   2-[(5R)-1-acetyl-3-(2-fluoro-5-methylphenyl)-5-phenyl-4,5-dihydro-1H-pyrazol-5-yl]ethyl    dihydrogen phosphate;-   2-[(5R)-1-acetyl-3-(2-fluoro-5-methylphenyl)-5-phenyl-4,5-dihydro-1H-pyrazol-5-yl]propan-1-ol;-   2-[(5R)-1-acetyl-3-(2-fluoro-5-methylphenyl)-5-phenyl-4,5-dihydro-1H-pyrazol-5-yl]propyl    dihydrogen phosphate;-   2-[(5R)-1-acetyl-3-(2-fluoro-5-methylphenyl)-5-phenyl-4,5-dihydro-1H-pyrazol-5-yl]butan-1-ol;-   2-[(5R)-1-acetyl-3-(2-fluoro-5-methylphenyl)-5-phenyl-4,5-dihydro-1H-pyrazol-5-yl]butyl    dihydrogen phosphate;-   3-[(5S)-1-acetyl-3-(5-bromo-2-fluorophenyl)-5-phenyl-4,5-dihydro-1H-pyrazol-5-yl]propan-1-ol;    and-   3-[(5S)-1-acetyl-3-(5-bromo-2-fluorophenyl)-5-phenyl-4,5-dihydro-1H-pyrazol-5-yl]propyl    dihydrogen phosphate;    or a pharmaceutically acceptable salt or stereoisomer thereof.

The compounds of the present invention may have asymmetric centers,chiral axes, and chiral planes (as described in: E. L. Eliel and S. H.Wilen, Stereochemistry of Carbon Compounds, John Wiley & Sons, New York,1994, pages 1119-1190), and occur as racemates, racemic mixtures, and asindividual diastereomers, with all possible isomers and mixturesthereof, including optical isomers, all such stereoisomers beingincluded in the present invention. In addition, the compounds disclosedherein may exist as tautomers and both tautomeric forms are intended tobe encompassed by the scope of the invention, even though only onetautomeric structure is depicted.

When any variable (e.g. R⁷, R⁸, R^(b), etc.) occurs more than one timein any constituent, its definition on each occurrence is independent atevery other occurrence. Also, combinations of substituents and variablesare permissible only if such combinations result in stable compounds.Lines drawn into the ring systems from substituents represent that theindicated bond may be attached to any of the substitutable ring atoms.If the ring system is polycyclic, it is intended that the bond beattached to any of the suitable carbon atoms on the proximal ring only.

It is understood that substituents and substitution patterns on thecompounds of the instant invention can be selected by one of ordinaryskill in the art to provide compounds that are chemically stable andthat can be readily synthesized by techniques known in the art, as wellas those methods set forth below, from readily available startingmaterials. If a substituent is itself substituted with more than onegroup, it is understood that these multiple groups may be on the samecarbon or on different carbons, so long as a stable structure results.The phrase “optionally substituted with one or more substituents” shouldbe taken to be equivalent to the phrase “optionally substituted with atleast one substituent” and in such cases the preferred embodiment willhave from zero to three substituents.

As used herein, “alkyl” is intended to include both branched andstraight-chain saturated aliphatic hydrocarbon groups having thespecified number of carbon atoms. For example, C₁-C₁₀, as in “C₁-C₁₀alkyl” is defined to include groups having 1, 2, 3, 4, 5, 6, 7, 8, 9 or10 carbons in a linear or branched arrangement. For example, “C₁-C₁₀alkyl” specifically includes methyl, ethyl, n-propyl, i-propyl, n-butyl,t-butyl, i-butyl, pentyl, hexyl, heptyl, octyl, nonyl, decyl, and so on.The term “cycloalkyl” means a monocyclic saturated aliphatic hydrocarbongroup having the specified number of carbon atoms. For example,“cycloalkyl” includes cyclopropyl, methyl-cyclopropyl,2,2-dimethyl-cyclobutyl, 2-ethyl-cyclopentyl, cyclohexyl, and so on. Inan embodiment of the invention the term “cycloalkyl” includes the groupsdescribed immediately above and further includes monocyclic unsaturatedaliphatic hydrocarbon groups. For example, “cycloalkyl” as defined inthis embodiment includes cyclopropyl, methyl-cyclopropyl,2,2-dimethyl-cyclobutyl, 2-ethyl-cyclopentyl, cyclohexyl, cyclopentenyl,cyclobutenyl and so on.

The term “alkylene” means a hydrocarbon diradical group having thespecified number of carbon atoms. For example, “alkylene” includes—CH₂—, —CH₂CH₂— and the like.

When used in the phrases “C₁-C₆ aralkyl” and “C₁-C₆ heteroaralkyl” theterm “C₁-C₆” refers to the alkyl portion of the moiety and does notdescribe the number of atoms in the aryl and heteroaryl portion of themoiety.

“Alkoxy” represents either a cyclic or non-cyclic alkyl group ofindicated number of carbon atoms attached through an oxygen bridge.“Alkoxy” therefore encompasses the definitions of alkyl and cycloalkylabove.

If no number of carbon atoms is specified, the term “alkenyl” refers toa non-aromatic hydrocarbon radical, straight, branched or cyclic,containing from 2 to 10 carbon atoms and at least one carbon to carbondouble bond. Preferably one carbon to carbon double bond is present, andup to four non-aromatic carbon-carbon double bonds may be present. Thus,“C₂-C₆ alkenyl” means an alkenyl radical having from 2 to 6 carbonatoms. Alkenyl groups include ethenyl, propenyl, butenyl,2-methylbutenyl and cyclohexenyl. The straight, branched or cyclicportion of the alkenyl group may contain double bonds and may besubstituted if a substituted alkenyl group is indicated.

The term “alkynyl” refers to a hydrocarbon radical straight, branched orcyclic, containing from 2 to 10 carbon atoms and at least one carbon tocarbon triple bond. Up to three carbon-carbon triple bonds may bepresent. Thus, “C₂-C₆ alkynyl” means an alkynyl radical having from 2 to6 carbon atoms. Alkynyl groups include ethynyl, propynyl, butynyl,3-methylbutynyl and so on. The straight, branched or cyclic portion ofthe alkynyl group may contain triple bonds and may be substituted if asubstituted alkynyl group is indicated.

In certain instances, substituents may be defined with a range ofcarbons that includes zero, such as (C₀-C₆)alkylene-aryl. If aryl istaken to be phenyl, this definition would include phenyl itself as wellas —CH₂Ph, —CH₂CH₂Ph, CH(CH₃)CH₂CH(CH₃)Ph, and so on.

As used herein, “aryl” is intended to mean any stable monocyclic orbicyclic carbon ring of up to 7 atoms in each ring, wherein at least onering is aromatic. Examples of such aryl elements include phenyl,naphthyl, tetrahydronaphthyl, indanyl and biphenyl. In cases where thearyl substituent is bicyclic and one ring is non-aromatic, it isunderstood that attachment is via the aromatic ring.

The term heteroaryl, as used herein, represents a stable monocyclic orbicyclic ring of up to 7 atoms in each ring, wherein at least one ringis aromatic and contains from 1 to 4 heteroatoms selected from the groupconsisting of O, N and S. Heteroaryl groups within the scope of thisdefinition include but are not limited to: acridinyl, carbazolyl,cinnolinyl, quinoxalinyl, pyrrazolyl, indolyl, benzotriazolyl, furanyl,thienyl, benzothienyl, benzofuranyl, quinolinyl, isoquinolinyl,oxazolyl, isoxazolyl, indolyl, pyrazinyl, pyridazinyl, pyridinyl,pyrimidinyl, pyrrolyl, tetrahydroquinoline. As with the definition ofheterocycle below, “heteroaryl” is also understood to include theN-oxide derivative of any nitrogen-containing heteroaryl. In cases wherethe heteroaryl substituent is bicyclic and one ring is non-aromatic orcontains no heteroatoms, it is understood that attachment is via thearomatic ring or via the heteroatom containing ring, respectively.

The term “heterocycle” or “heterocyclyl” as used herein is intended tomean a 3- to 10-membered aromatic or nonaromatic heterocycle containingfrom 1 to 4 heteroatoms selected from the group consisting of O, N andS, and includes bicyclic groups. “Heterocyclyl” therefore includes theabove mentioned heteroaryls, as well as dihydro and tetrathydro analogsthereof. Further examples of “heterocyclyl” include, but are not limitedto the following: azetidinyl, benzoimidazolyl, benzofuranyl,benzofurazanyl, benzopyrazolyl, benzotriazolyl, benzothiophenyl,benzoxazolyl, carbazolyl, carbolinyl, cinnolinyl, furanyl, imidazolyl,indolinyl, indolyl, indolazinyl, indazolyl, isobenzofuranyl, isoindolyl,isoquinolyl, isothiazolyl, isoxazolyl, naphthpyridinyl, oxadiazolyl,oxazolyl, oxazoline, isoxazoline, oxetanyl, pyranyl, pyrazinyl,pyrazolyl, pyridazinyl, pyridopyridinyl, pyridazinyl, pyridyl,pyrimidyl, pyrrolyl, quinazolinyl, quinolyl, quinoxalinyl,tetrahydropyranyl, tetrahydrothiopyranyl, tetrahydroisoquinolinyl,tetrazolyl, tetrazolopyridyl, thiadiazolyl, thiazolyl, thienyl,triazolyl, 1,4-dioxanyl, hexahydroazepinyl, piperazinyl, piperidinyl,pyridin-2-onyl, pyrrolidinyl, morpholinyl, thiomorpholinyl,dihydrobenzoimidazolyl, dihydrobenzofuranyl, dihydrobenzothiophenyl,dihydrobenzoxazolyl, dihydrofuranyl, dihydroimidazolyl, dihydroindolyl,dihydroisooxazolyl, dihydroisothiazolyl, dihydrooxadiazolyl,dihydrooxazolyl, dihydropyrazinyl, dihydropyrazolyl, dihydropyridinyl,dihydropyrimidinyl, dihydropyrrolyl, dihydroquinolinyl,dihydrotetrazolyl, dihydrothiadiazolyl, dihydrothiazolyl,dihydrothienyl, dihydrotriazolyl, dihydroazetidinyl,methylenedioxybenzoyl, tetrahydrofuranyl, and tetrahydrothienyl, andN-oxides thereof. Attachment of a heterocyclyl substituent can occur viaa carbon atom or via a heteroatom.

In an embodiment, the term “heterocycle” or “heterocyclyl” as usedherein is intended to mean a 5- to 10-membered aromatic or nonaromaticheterocycle containing from 1 to 4 heteroatoms selected from the groupconsisting of O, N and S, and includes bicyclic groups. “Heterocyclyl”in this embodiment therefore includes the above mentioned heteroaryls,as well as dihydro and tetrathydro analogs thereof. Further examples of“heterocyclyl” include, but are not limited to the following:benzoimidazolyl, benzofuranyl, benzofurazanyl, benzopyrazolyl,benzotriazolyl, benzothiophenyl, benzoxazolyl, carbazolyl, carbolinyl,cinnolinyl, furanyl, imidazolyl, indolinyl, indolyl, indolazinyl,indazolyl, isobenzofuranyl, isoindolyl, isoquinolyl, isothiazolyl,isoxazolyl, naphthpyridinyl, oxadiazolyl, oxazolyl, oxazoline,isoxazoline, oxetanyl, pyranyl, pyrazinyl, pyrazolyl, pyridazinyl,pyridopyridinyl, pyridazinyl, pyridyl, pyrimidyl, pyrrolyl,quinazolinyl, quinolyl, quinoxalinyl, tetrahydropyranyl,tetrahydrothiopyranyl, tetrahydroisoquinolinyl, tetrazolyl,tetrazolopyridyl, thiadiazolyl, thiazolyl, thienyl, triazolyl,azetidinyl, 1,4-dioxanyl, hexahydroazepinyl, piperazinyl, piperidinyl,pyridin-2-onyl, pyrrolidinyl, morpholinyl, thiomorpholinyl,dihydrobenzoimidazolyl, dihydrobenzofuranyl, dihydrobenzothiophenyl,dihydrobenzoxazolyl, dihydrofuranyl, dihydroimidazolyl, dihydroindolyl,dihydroisooxazolyl, dihydroisothiazolyl, dihydrooxadiazolyl,dihydrooxazolyl, dihydropyrazinyl, dihydropyrazolyl, dihydropyridinyl,dihydropyrimidinyl, dihydropyrrolyl, dihydroquinolinyl,dihydrotetrazolyl, dihydrothiadiazolyl, dihydrothiazolyl,dihydrothienyl, dihydrotriazolyl, dihydroazetidinyl,methylenedioxybenzoyl, tetrahydrofuranyl, and tetrahydrothienyl, andN-oxides thereof. Attachment of a heterocyclyl substituent can occur viaa carbon atom or via a heteroatom.

In another embodiment, heterocycle is selected from 2-azepinone,benzimidazolyl, 2-diazapinone, imidazolyl, 2-imidazolidinone, indolyl,isoquinolinyl, morpholinyl, piperidyl, piperazinyl, pyridyl,pyrrolidinyl, 2-piperidinone, 2-pyrimidinone, 2-pyrollidinone,quinolinyl, tetrahydrofuryl, tetrahydroisoquinolinyl, and thienyl.

As appreciated by those of skill in the art, “halo” or “halogen” as usedherein is intended to include chloro, fluoro, bromo and iodo.

The alkyl, alkenyl, alkynyl, cycloalkyl, aryl, heteroaryl andheterocyclyl substituents may be substituted or unsubstituted, unlessspecifically defined otherwise. For example, a (C₁-C₆)alkyl may besubstituted with one, two or three substituents selected from OH, oxo,halogen, alkoxy, dialkylamino, or heterocyclyl, such as morpholinyl,piperidinyl, and so on. In this case, if one substituent is oxo and theother is OH, the following are included in the definition:—C═O)CH₂CH(OH)CH₃, —(C═O)OH, —CH₂(OH)CH₂CH(O), and so on.

The moiety formed when, in the definition of R³ and R⁴ on the samecarbon atom are combined to form —(CH₂)_(u)— is illustrated by thefollowing:

In addition, such cyclic moieties may optionally include aheteroatom(s). Examples of such heteroatom-containing cyclic moietiesinclude, but are not limited to:

In certain instances, R⁹ and R¹⁰ are defined such that they can be takentogether with the nitrogen to which they are attached to form amonocyclic or bicyclic heterocycle with 5-7 members in each ring andoptionally containing, in addition to the nitrogen, one or twoadditional heteroatoms selected from N, O and S, said heterocycleoptionally substituted with one or more substituents selected from R¹¹.Examples of the heterocycles that can thus be formed include, but arenot limited to the following, keeping in mind that the heterocycle isoptionally substituted with one or more (and preferably one, two orthree) substituents chosen from R¹¹:

In certain instances, two R^(c)s are defined such that they can be takentogether with the phosphorous to which they are attached to form amonocyclic heterocycle with 5-7 members in the ring, said heterocycleoptionally substituted with one or more substituents selected fromR^(b). Examples of the heterocycles that can thus be formed include, butare not limited to the following, keeping in mind that the heterocycleis optionally substituted with one or more (and preferably one or two)substituents chosen from R^(b):

In an embodiment, n is 1, 2 or 3.

In an embodiment, R¹ is selected from (C═O)C₁-C₁₀ alkyl, (C═O)aryl,(C═O)C₃-C₈ cycloalkyl and —(C═O)heteroaryl, optionally substituted withone to three substituents selected from R⁷. In another aspect of thisembodiment, R¹ is (C═O)C₁-C₁₀ alkyl.

In an embodiment, R² is selected from aryl, optionally substituted withone to three substituents selected from R⁷. In another aspect of thisembodiment, R² is phenyl, optionally substituted with one to threesubstituents selected from halo and C₁-C₆ alkyl.

In an embodiment, R³ and R⁴ are H.

In an embodiment, R⁵ is selected from aryl, optionally substituted withone to three substituents selected from R¹⁰. In another aspect of thisembodiment, R⁵ is phenyl, optionally substituted with one to threesubstituents selected from halo and —OH. In another aspect of thisembodiment, R⁵ is phenyl, optionally substituted with one to threesubstituents selected from halo.

In an embodiment, R^(p) is —P(O)(OH)₂.

In an embodiment of the compound of the formula IV, R¹¹ is C₁-C₆ alkyl.In a further embodiment of the compound of the formula IV, R¹¹ ismethyl.

In an embodiment of the compound of the formula IV, R^(7a) and R^(7c)are independently selected from C₁-C₆ alkyl, halo and OH.

In an embodiment of the compound of the formula IV, R^(7b) is F.

In an embodiment of the compound of the formula IV, n is 1, 2 or 3.

Included in the instant invention is the free form of compounds ofFormula I, as well as the pharmaceutically acceptable salts andstereoisomers thereof. Some of the specific compounds exemplified hereinare the protonated salts of amine compounds. The term “free form” refersto the amine compounds in non-salt form. The encompassedpharmaceutically acceptable salts not only include the salts exemplifiedfor the specific compounds described herein, but also all the typicalpharmaceutically acceptable salts of the free form of compounds ofFormula I. The free form of the specific salt compounds described may beisolated using techniques known in the art. For example, the free formmay be regenerated by treating the salt with a suitable dilute aqueousbase solution such as dilute aqueous NaOH, potassium carbonate, ammoniaand sodium bicarbonate. The free forms may differ from their respectivesalt forms somewhat in certain physical properties, such as solubilityin polar solvents, but the acid and base salts are otherwisepharmaceutically equivalent to their respective free forms for purposesof the invention.

The pharmaceutically acceptable salts of the instant compounds can besynthesized from the compounds of this invention which contain a basicor acidic moiety by conventional chemical methods. Generally, the saltsof the basic compounds are prepared either by ion exchangechromatography or by reacting the free base with stoichiometric amountsor with an excess of the desired salt-forming inorganic or organic acidin a suitable solvent or various combinations of solvents. Similarly,the salts of the acidic compounds are formed by reactions with theappropriate inorganic or organic base.

Thus, pharmaceutically acceptable salts of the compounds of thisinvention include the conventional non-toxic salts of the compounds ofthis invention as formed by reacting a basic instant compound with aninorganic or organic acid. For example, conventional non-toxic saltsinclude those derived from inorganic acids such as hydrochloric,hydrobromic, sulfuric, sulfamic, phosphoric, nitric and the like, aswell as salts prepared from organic acids such as acetic, propionic,succinic, glycolic, stearic, lactic, malic, tartaric, citric, ascorbic,pamoic, maleic, hydroxymaleic, phenylacetic, glutamic, benzoic,salicylic, sulfanilic, 2-acetoxy-benzoic, fumaric, toluenesulfonic,methanesulfonic, ethane disulfonic, oxalic, isethionic, trifluoroaceticand the like.

When the compound of the present invention is acidic, suitable“pharmaceutically acceptable salts” refers to salts prepared formpharmaceutically acceptable non-toxic bases including inorganic basesand organic bases. The mono- and dibasic salts of the phosphate estersmoieties of the instant compounds are particularly included in theinstant invention. Salts derived from inorganic bases include aluminum,ammonium, calcium, copper, ferric, ferrous, lithium, magnesium, manganicsalts, manganous, potassium, sodium, zinc and the like. Particularlypreferred are the ammonium, calcium, magnesium, potassium and sodiumsalts. Salts derived from pharmaceutically acceptable organic non-toxicbases include salts of primary, secondary and tertiary amines,substituted amines including naturally occurring substituted amines,cyclic amines and basic ion exchange resins, such as arginine, betainecaffeine, choline, N,N¹-dibenzylethylenediamine, diethylamin,2-diethylaminoethanol, 2-dimethylaminoethanol, ethanolamine,ethylenediamine, N-ethylmorpholine, N-ethylpiperidine, glucamine,glucosamine, histidine, hydrabamine, isopropylamine, lysine,methylglucamine, morpholine, piperazine, piperidine, polyamine resins,procaine, purines, theobromine, triethylamine, trimethylaminetripropylamine, tromethamine and the like. When the compound of thepresent invention is acidic, the term “free form” refers to the compoundin its non-salt form, such that the acidic functionality is stillprotonated.

The preparation of the pharmaceutically acceptable salts described aboveand other typical pharmaceutically acceptable salts is more fullydescribed by Berg et al., “Pharmaceutical Salts,” J. Pharm. Sci.,1977:66:1-19.

It will also be noted that the compounds of the present invention maypotentially be internal salts or zwitterions, since under physiologicalconditions a deprotonated acidic moiety in the compound, such as acarboxyl group, may be anionic, and this electronic charge might then bebalanced off internally against the cationic charge of a protonated oralkylated basic moiety, such as a quaternary nitrogen atom. An isolatedcompound having internally balance charges, and thus not associated witha intermolecular counterion, may also be considered the “free form” of acompound.

The compounds of this invention may be prepared by employing reactionsas shown in the following schemes, in addition to other standardmanipulations that are known in the literature or exemplified in theexperimental procedures. The illustrative schemes below, therefore, arenot limited by the compounds listed or by any particular substituentsemployed for illustrative purposes. Substituent numbering as shown inthe schemes does not necessarily correlate to that used in the claimsand often, for clarity, a single substituent is shown attached to thecompound where multiple substituents are allowed under the definitionsof Formula I hereinabove.

Schemes

As shown in Scheme A, reaction of a suitably substitutedphenylhydroxamic ester A-1 with a suitably substituted acetylene A-2provides the benzoyl alkyne intermediate A-3. Copper mediated couplingof a suitably substituted phenyl group provides intermediate A-4.Intermediate A-4 can then undergo reaction with hydrazine in thepresence of an acyl chloride to provide the N-acyldihydropyrazole A-5.Deprotection of the terminal hydroxyl group provides the instantcompound A-6.

Scheme B shows an analogous series of reactions using an alternativehydroxyl protecting group to prepare the instant compound B-5. TheScheme also illustrates formation of the phosphate prodrug ester B-6.

Schemes C-G illustrate incorporation of the other prodrug moieties ofthe instant compounds.

Utilities

The compounds of the invention find use in a variety of applications. Aswill be appreciated by those skilled in the art, mitosis may be alteredin a variety of ways; that is, one can affect mitosis either byincreasing or decreasing the activity of a component in the mitoticpathway. Stated differently, mitosis may be affected (e.g., disrupted)by disturbing equilibrium, either by inhibiting or activating certaincomponents. Similar approaches may be used to alter meiosis.

In an embodiment, the compounds of the invention are used to modulatemitotic spindle formation, thus causing prolonged cell cycle arrest inmitosis. By “modulate” herein is meant altering mitotic spindleformation, including increasing and decreasing spindle formation. By“mitotic spindle formation” herein is meant organization of microtubulesinto bipolar structures by mitotic kinesins. By “mitotic spindledysfunction” herein is meant mitotic arrest and monopolar spindleformation.

The compounds of the invention are useful to bind to and/or modulate theactivity of a mitotic kinesin. In an embodiment, the mitotic kinesin isa member of the bimC subfamily of mitotic kinesins (as described in U.S.Pat. No. 6,284,480, column 5). In a further embodiment, the mitotickinesin is human KSP, although the activity of mitotic kinesins fromother organisms may also be modulated by the compounds of the presentinvention. In this context, modulate means either increasing ordecreasing spindle pole separation, causing malformation, i.e.,splaying, of mitotic spindle poles, or otherwise causing morphologicalperturbation of the mitotic spindle. Also included within the definitionof KSP for these purposes are avariants and/or fragments of KSP. Inaddition, other mitotic kinesins may be inhibited by the compounds ofthe present invention.

The compounds of the invention are used to treat cellular proliferationdiseases. Disease states which can be treated by the methods andcompositions provided herein include, but are not limited to, cancer(further discussed below), autoimmune disease, arthritis, graftrejection, inflammatory bowel disease, proliferation induced aftermedical procedures, including, but not limited to, surgery, angioplasty,and the like. It is appreciated that in some cases the cells may not bein a hyper- or hypoproliferation state (abnormal state) and stillrequire treatment. For example, during wound healing, the cells may beproliferating “normally”, but proliferation enhancement may be desired.Similarly, as discussed above, in the agriculture arena, cells may be ina “normal” state, but proliferation modulation may be desired to enhancea crop by directly enhancing growth of a crop, or by inhibiting thegrowth of a plant or organism which adversely affects the crop. Thus, inone embodiment, the invention herein includes application to cells orindividuals which are afflicted or may eventually become afflicted withany one of these disorders or states.

The compounds, compositions and methods provided herein are particularlydeemed useful for the treatment of cancer including solid tumors such asskin, breast, brain, cervical carcinomas, testicular carcinomas, etc. Inparticular, cancers that may be treated by the compounds, compositionsand methods of the invention include, but are not limited to: Cardiac:sarcoma (angiosarcoma, fibrosarcoma, rhabdomyosarcoma, liposarcoma),myxoma, rhabdomyoma, fibroma, lipoma and teratoma; Lung: bronchogeniccarcinoma (squamous cell, undifferentiated small cell, undifferentiatedlarge cell, adenocarcinoma), alveolar (bronchiolar) carcinoma, bronchialadenoma, sarcoma, lymphoma, chondromatous hamartoma, mesothelioma;Gastrointestinal: esophagus (squamous cell carcinoma, adenocarcinoma,leiomyosarcoma, lymphoma), stomach (carcinoma, lymphoma,leiomyosarcoma), pancreas (ductal adenocarcinoma, insulinoma,glucagonoma, gastrinoma, carcinoid tumors, vipoma), small bowel(adenocarcinoma, lymphoma, carcinoid tumors, Karposi's sarcoma,leiomyoma, hemangioma, lipoma, neurofibroma, fibroma), large bowel(adenocarcinoma, tubular adenoma, villous adenoma, hamartoma,leiomyoma); Genitourinary tract: kidney (adenocarcinoma, Wilm's tumor[nephroblastoma], lymphoma, leukemia), bladder and urethra (squamouscell carcinoma, transitional cell carcinoma, adenocarcinoma), prostate(adenocarcinoma, sarcoma), testis (seminoma, teratoma, embryonalcarcinoma, teratocarcinoma, choriocarcinoma, sarcoma, interstitial cellcarcinoma, fibroma, fibroadenoma, adenomatoid tumors, lipoma); Liver:hepatoma (hepatocellular carcinoma), cholangiocarcinoma, hepatoblastoma,angiosarcoma, hepatocellular adenoma, hemangioma; Bone: osteogenicsarcoma (osteosarcoma), fibrosarcoma, malignant fibrous histiocytoma,chondrosarcoma, Ewing's sarcoma, malignant lymphoma (reticulum cellsarcoma), multiple myeloma, malignant giant cell tumor chordoma,osteochronfroma (osteocartilaginous exostoses), benign chondroma,chondroblastoma, chondromyxofibroma, osteoid osteoma and giant celltumors; Nervous system: skull (osteoma, hemangioma, granuloma, xanthoma,osteitis deformans), meninges (meningioma, meningiosarcoma,gliomatosis), brain (astrocytoma, medulloblastoma, glioma, ependymoma,germinoma [pinealoma], glioblastoma multiform, oligodendroglioma,schwannoma, retinoblastoma, congenital tumors), spinal cordneurofibroma, meningioma, glioma, sarcoma); Gynecological: uterus(endometrial carcinoma), cervix (cervical carcinoma, pre-tumor cervicaldysplasia), ovaries (ovarian carcinoma [serous cystadenocarcinoma,mucinous cystadenocarcinoma, unclassified carcinoma], granulosa-thecalcell tumors, Sertoli-Leydig cell tumors, dysgerminoma, malignantteratoma), vulva (squamous cell carcinoma, intraepithelial carcinoma,adenocarcinoma, fibrosarcoma, melanoma), vagina (clear cell carcinoma,squamous cell carcinoma, botryoid sarcoma (embryonal rhabdomyosarcoma),fallopian tubes (carcinoma); Hematologic: blood (myeloid leukemia [acuteand chronic], acute lymphoblastic leukemia, chronic lymphocyticleukemia, myeloproliferative diseases, multiple myeloma, myelodysplasticsyndrome), Hodgkin's disease, non-Hodgkin's lymphoma [malignantlymphoma]; Skin: malignant melanoma, basal cell carcinoma, squamous cellcarcinoma, Karposi's sarcoma, moles dysplastic nevi, lipoma, angioma,dermatofibroma, keloids, psoriasis; and Adrenal glands: neuroblastoma.Thus, the term “cancerous cell” as provided herein, includes a cellafflicted by any one of the above-identified conditions.

The compounds of the instant invention may also be useful as antifungalagents, by modulating the activity of the fungal members of the bimCkinesin subgroup, as is described in U.S. Pat. No. 6,284,480.

The compounds of this invention may be administered to mammals,preferably humans, either alone or in combination with pharmaceuticallyacceptable carriers, excipients or diluents, in a pharmaceuticalcomposition, according to standard pharmaceutical practice. Thecompounds can be administered orally or parenterally, including theintravenous, intramuscular, intraperitoneal, subcutaneous, rectal andtopical routes of administration.

The pharmaceutical compositions containing the active ingredient may bein a form suitable for oral use, for example, as tablets, troches,lozenges, aqueous or oily suspensions, dispersible powders or granules,emulsions, hard or soft capsules, or syrups or elixirs. Compositionsintended for oral use may be prepared according to any method known tothe art for the manufacture of pharmaceutical compositions and suchcompositions may contain one or more agents selected from the groupconsisting of sweetening agents, flavoring agents, coloring agents andpreserving agents in order to provide pharmaceutically elegant andpalatable preparations. Tablets contain the active ingredient inadmixture with non-toxic pharmaceutically acceptable excipients whichare suitable for the manufacture of tablets. These excipients may be forexample, inert diluents, such as calcium carbonate, sodium carbonate,lactose, calcium phosphate or sodium phosphate; granulating anddisintegrating agents, for example, microcrystalline cellulose, sodiumcrosscarmellose, corn starch, or alginic acid; binding agents, forexample starch, gelatin, polyvinyl-pyrrolidone or acacia, andlubricating agents, for example, magnesium stearate, stearic acid ortalc. The tablets may be uncoated or they may be coated by knowntechniques to mask the unpleasant taste of the drug or delaydisintegration and absorption in the gastrointestinal tract and therebyprovide a sustained action over a longer period. For example, a watersoluble taste masking material such as hydroxypropyl-methylcellulose orhydroxypropylcellulose, or a time delay material such as ethylcellulose, cellulose acetate butyrate may be employed.

Formulations for oral use may also be presented as hard gelatin capsuleswherein the active ingredient is mixed with an inert solid diluent, forexample, calcium carbonate, calcium phosphate or kaolin, or as softgelatin capsules wherein the active ingredient is mixed with watersoluble carrier such as polyethyleneglycol or an oil medium, for examplepeanut oil, liquid paraffin, or olive oil.

Aqueous suspensions contain the active material in admixture withexcipients suitable for the manufacture of aqueous suspensions. Suchexcipients are suspending agents, for example sodiumcarboxymethylcellulose, methylcellulose, hydroxypropylmethyl-cellulose,sodium alginate, polyvinyl-pyrrolidone, gum tragacanth and gum acacia;dispersing or wetting agents may be a naturally-occurring phosphatide,for example lecithin, or condensation products of an alkylene oxide withfatty acids, for example polyoxyethylene stearate, or condensationproducts of ethylene oxide with long chain aliphatic alcohols, forexample heptadecaethyleneoxycetanol, or condensation products ofethylene oxide with partial esters derived from fatty acids and ahexitol such as polyoxyethylene sorbitol monooleate, or condensationproducts of ethylene oxide with partial esters derived from fatty acidsand hexitol anhydrides, for example polyethylene sorbitan monooleate.The aqueous suspensions may also contain one or more preservatives, forexample ethyl, or n-propyl p-hydroxybenzoate, one or more coloringagents, one or more flavoring agents, and one or more sweetening agents,such as sucrose, saccharin or aspartame.

Oily suspensions may be formulated by suspending the active ingredientin a vegetable oil, for example arachis oil, olive oil, sesame oil orcoconut oil, or in mineral oil such as liquid paraffin. The oilysuspensions may contain a thickening agent, for example beeswax, hardparaffin or cetyl alcohol. Sweetening agents such as those set forthabove, and flavoring agents may be added to provide a palatable oralpreparation. These compositions may be preserved by the addition of ananti-oxidant such as butylated hydroxyanisol or alpha-tocopherol.

Dispersible powders and granules suitable for preparation of an aqueoussuspension by the addition of water provide the active ingredient inadmixture with a dispersing or wetting agent, suspending agent and oneor more preservatives. Suitable dispersing or wetting agents andsuspending agents are exemplified by those already mentioned above.Additional excipients, for example sweetening, flavoring and coloringagents, may also be present. These compositions may be preserved by theaddition of an anti-oxidant such as ascorbic acid.

The pharmaceutical compositions of the invention may also be in the formof an oil-in-water emulsions. The oily phase may be a vegetable oil, forexample olive oil or arachis oil, or a mineral oil, for example liquidparaffin or mixtures of these. Suitable emulsifying agents may benaturally occurring phosphatides, for example soy bean lecithin, andesters or partial esters derived from fatty acids and hexitolanhydrides, for example sorbitan monooleate, and condensation productsof the said partial esters with ethylene oxide, for examplepolyoxyethylene sorbitan monooleate. The emulsions may also containsweetening, flavoring agents, preservatives and antioxidants.

Syrups and elixirs may be formulated with sweetening agents, for exampleglycerol, propylene glycol, sorbitol or sucrose. Such formulations mayalso contain a demulcent, a preservative, flavoring and coloring agentsand antioxidant.

The pharmaceutical compositions may be in the form of a sterileinjectable aqueous solutions. Among the acceptable vehicles and solventsthat may be employed are water, Ringer's solution,polyethyleneglycol/water solution and isotonic sodium chloride solution.

The sterile injectable preparation may also be a sterile injectableoil-in-water microemulsion where the active ingredient is dissolved inthe oily phase. For example, the active ingredient may be firstdissolved in a mixture of soybean oil and lecithin. The oil solutionthen introduced into a water and glycerol mixture and processed to forma microemulation.

The injectable solutions or microemulsions may be introduced into apatient's blood stream by local bolus injection. Alternatively, it maybe advantageous to administer the solution or microemulsion in such away as to maintain a constant circulating concentration of the instantcompound. In order to maintain such a constant concentration, acontinuous intravenous delivery device may be utilized. An example ofsuch a device is the Deltec CADD-PLUS™ model 5400 intravenous pump.

The pharmaceutical compositions may be in the form of a sterileinjectable aqueous or oleagenous suspension for intramuscular andsubcutaneous administration. This suspension may be formulated accordingto the known art using those suitable dispersing or wetting agents andsuspending agents which have been mentioned above. The sterileinjectable preparation may also be a sterile injectable solution orsuspension in a non-toxic parenterally acceptable diluent or solvent,for example as a solution in 1,3-butane diol. In addition, sterile,fixed oils are conventionally employed as a solvent or suspendingmedium. For this purpose any bland fixed oil may be employed includingsynthetic mono- or diglycerides. In addition, fatty acids such as oleicacid find use in the preparation of injectables.

Compounds of Formula I may also be administered in the form ofsuppositories for rectal administration of the drug. These compositionscan be prepared by mixing the drug with a suitable non-irritatingexcipient which is solid at ordinary temperatures but liquid at therectal temperature and will therefore melt in the rectum to release thedrug. Such materials include cocoa butter, glycerinated gelatin,hydrogenated vegetable oils, mixtures of polyethylene glycols of variousmolecular weights and fatty acid esters of polyethylene glycol.

For topical use, creams, ointments, jellies, solutions or suspensions,etc., containing the compound of Formula I are employed. (For purposesof this application, topical application shall include mouth washes andgargles.)

The compounds for the present invention can be administered inintranasal form via topical use of suitable intranasal vehicles anddelivery devices, or via transdermal routes, using those forms oftransdermal skin patches well known to those of ordinary skill in theart. To be administered in the form of a transdermal delivery system,the dosage administration will, of course, be continuous rather thanintermittent throughout the dosage regimen. Compounds of the presentinvention may also be delivered as a suppository employing bases such ascocoa butter, glycerinated gelatin, hydrogenated vegetable oils,mixtures of polyethylene glycols of various molecular weights and fattyacid esters of polyethylene glycol.

When a compound according to this invention is administered into a humansubject, the daily dosage will normally be determined by the prescribingphysician with the dosage generally varying according to the age,weight, sex and response of the individual patient, as well as theseverity of the patient's symptoms.

In one exemplary application, a suitable amount of compound isadministered to a mammal undergoing treatment for cancer. Administrationoccurs in an amount between about 0.1 mg/kg of body weight to about 60mg/kg of body weight per day, preferably of between 0.5 mg/kg of bodyweight to about 40 mg/kg of body weight per day.

The instant compounds are also useful in combination with knowntherapeutic agents and anti-cancer agents. For example, instantcompounds are useful in combination with known anti-cancer agents.Combinations of the presently disclosed compounds with other anti-canceror chemotherapeutic agents are within the scope of the invention.Examples of such agents can be found in Cancer Principles and Practiceof Oncology by V. T. Devita and S. Hellman (editors), 6^(th) edition(Feb. 15, 2001), Lippincott Williams & Wilkins Publishers. A person ofordinary skill in the art would be able to discern which combinations ofagents would be useful based on the particular characteristics of thedrugs and the cancer involved. Such anti-cancer agents include, but arenot limited to, the following: estrogen receptor modulators, androgenreceptor modulators, retinoid receptor modulators, cytotoxic/cytostaticagents, antiproliferative agents, prenyl-protein transferase inhibitors,HMG-CoA reductase inhibitors and other angiogenesis inhibitors,inhibitors of cell proliferation and survival signaling, apoptosisinducing agents and agents that interfere with cell cycle checkpoints.The instant compounds are particularly useful when co-administered withradiation therapy.

In an embodiment, the instant compounds are also useful in combinationwith known anti-cancer agents including the following: estrogen receptormodulators, androgen receptor modulators, retinoid receptor modulators,cytotoxic agents, antiproliferative agents, prenyl-protein transferaseinhibitors, HMG-CoA reductase inhibitors, HIV protease inhibitors,reverse transcriptase inhibitors, and other angiogenesis inhibitors.

“Estrogen receptor modulators” refers to compounds that interfere withor inhibit the binding of estrogen to the receptor, regardless ofmechanism. Examples of estrogen receptor modulators include, but are notlimited to, tamoxifen, raloxifene, idoxifene, LY353381, LY117081,toremifene, fulvestrant,4-[7-(2,2-dimethyl-1-oxopropoxy-4-methyl-2-[4-[2-(1-piperidinyl)ethoxy]phenyl]-2H-1-benzopyran-3-yl]-phenyl-2,2-dimethylpropanoate,4,4′-dihydroxybenzophenone-2,4-dinitrophenyl-hydrazone, and SH646.

“Androgen receptor modulators” refers to compounds which interfere orinhibit the binding of androgens to the receptor, regardless ofmechanism. Examples of androgen receptor modulators include finasterideand other 5α-reductase inhibitors, nilutamide, flutamide, bicalutamide,liarozole, and abiraterone acetate.

“Retinoid receptor modulators” refers to compounds which interfere orinhibit the binding of retinoids to the receptor, regardless ofmechanism. Examples of such retinoid receptor modulators includebexarotene, tretinoin, 13-cis-retinoic acid, 9-cis-retinoic acid,α-difluoromethylornithine, ILX23-7553, trans-N-(4′-hydroxyphenyl)retinamide, and N-4-carboxyphenyl retinamide.

“Cytotoxic/cytostatic agents” refer to compounds which cause cell deathor inhibit cell proliferation primarily by interfering directly with thecell's functioning or inhibit or interfere with cell mytosis, includingalkylating agents, tumor necrosis factors, intercalators, hypoxiaactivatable compounds, microtubule inhibitors/microtubule-stabilizingagents, inhibitors of mitotic kinesins, inhibitors of kinases involvedin mitotic progression, antimetabolites; biological response modifiers;hormonal/anti-hormonal therapeutic agents, haematopoietic growthfactors, monoclonal antibody targeted therapeutic agents, topoisomeraseinhibitors, proteasome inhibitors and ubiquitin ligase inhibitors.

Examples of cytotoxic agents include, but are not limited to, sertenef,cachectin, ifosfamide, tasonermin, lonidamine, carboplatin, altretamine,prednimustine, dibromodulcitol, ranimustine, fotemustine, nedaplatin,oxaliplatin, temozolomide, heptaplatin, estramustine, improsulfantosilate, trofosfamide, nimustine, dibrospidium chloride, pumitepa,lobaplatin, satraplatin, profiromycin, cisplatin, irofulven,dexifosfamide, cis-aminedichloro(2-methyl-pyridine)platinum,benzylguanine, glufosfamide, GPX100, (trans, trans,trans)-bis-mu-(hexane-1,6-diamine)-mu-[diamine-platinum(II)]bis[diamine(chloro)platinum(II)]tetrachloride, diarizidinylspermine, arsenic trioxide,1-(11-dodecylamino-10-hydroxyundecyl)-3,7-dimethylxanthine, zorubicin,idarubicin, daunorubicin, bisantrene, mitoxantrone, pirarubicin,pinafide, valrubicin, amrubicin, antineoplaston,3′-deamino-3′-morpholino-13-deoxo-10-hydroxycaminomycin, annamycin,galarubicin, elinafide, MEN10755, and4-demethoxy-3-deamino-3-aziridinyl-4-methylsulphonyl-daunorubicin (seeWO 00/50032).

An example of a hypoxia activatable compound is tirapazamine.

Examples of proteasome inhibitors include but are not limited tolactacystin and bortezomib.

Examples of microtubule inhibitors/microtubule-stabilising agentsinclude paclitaxel, vindesine sulfate,3′,4′-didehydro-4′-deoxy-8′-norvincaleukoblastine, docetaxol, rhizoxin,dolastatin, mivobulin isethionate, auristatin, cemadotin, RPR109881,BMS184476, vinflunine, cryptophycin,2,3,4,5,6-pentafluoro-N-(3-fluoro-4-methoxyphenyl)benzene sulfonamide,anhydrovinblastine,N,N-dimethyl-L-valyl-L-valyl-N-methyl-L-valyl-L-prolyl-L-proline-t-butylamide,TDX258, the epothilones (see for example U.S. Pat. Nos. 6,284,781 and6,288,237) and BMS188797.

Some examples of topoisomerase inhibitors are topotecan, hycaptamine,irinotecan, rubitecan,6-ethoxypropionyl-3′,4′-O-exo-benzylidene-chartreusin,9-methoxy-N,N-dimethyl-5-nitropyrazolo[3,4,5-kl]acridine-2-(6H)propanamine,1-amino-9-ethyl-5-fluoro-2,3-dihydro-9-hydroxy-4-methyl-1H,12H-benzo[de]pyrano[3′,4′:b,7]-indolizino[1,2b]quinoline-10,13(9H,15H)dione, lurtotecan,7-[2-(N-isopropylamino)ethyl]-(20S)camptothecin, BNP1350, BNPI1100,BN80915, BN80942, etoposide phosphate, teniposide, sobuzoxane,2′-dimethylamino-2′-deoxy-etoposide, GL331,N-[2-(dimethylamino)ethyl]-9-hydroxy-5,6-dimethyl-6H-pyrido[4,3-b]carbazole-1-carboxamide,asulacrine,(5a,5aB,8aa,9b)-9-[2-[N-[2-(dimethylamino)ethyl]-N-methylamino]ethyl]-5-[4-hydroxy-3,5-dimethoxyphenyl]-5,5a,6,8,8a,9-hexohydrofuro(3′,4′:6,7)naphtho(2,3-d)-1,3-dioxol-6-one,2,3-(methylenedioxy)-5-methyl-7-hydroxy-8-methoxybenzo[c]-phenanthridinium,6,9-bis[(2-aminoethyl)amino]benzo[g]isoquinoline-5,10-dione,5-(3-aminopropylamino)-7,10-dihydroxy-2-(2-hydroxyethylaminomethyl)-6H-pyrazolo[4,5,1-de]acridin-6-one,N-[1-[2(diethylamino)ethylamino]-7-methoxy-9-oxo-9H-thioxanthen-4-ylmethyl]formamide,N-(2-(dimethylamino)ethyl)acridine-4-carboxamide,6-[[2-(dimethylamino)ethyl]amino]-3-hydroxy-7H-indeno[2,1-c]quinolin-7-one,and dimesna.

Examples of inhibitors of mitotic kinesins, and in particular the humanmitotic kinesin KSP, are described in PCT Publications WO 01/30768, WO01/98278, WO 03/050,064, WO 03/050,122, WO 03/049,527, WO 03/049,679, WO03/049,678 and WO 03/39460 and pending PCT Appl. Nos. US03/06403 (filedMar. 4, 2003), US03/15861 (filed May 19, 2003), US03/15810 (filed May19, 2003), US03/18482 (filed Jun. 12, 2003) and US03/18694 (filed Jun.12, 2003). In an embodiment inhibitors of mitotic kinesins include, butare not limited to inhibitors of KSP, inhibitors of MKLP1, inhibitors ofCENP-E, inhibitors of MCAK, inhibitors of Kif14, inhibitors of Mphosph1and inhibitors of Rab6-KIFL.

“Inhibitors of kinases involved in mitotic progression” include, but arenot limited to, inhibitors of aurora kinase, inhibitors of Polo-likekinases (PLK) (in particular inhibitors of PLK-1), inhibitors of bub-1and inhibitors of bub-R1.

“Antiproliferative agents” includes antisense RNA and DNAoligonucleotides such as G3139, ODN698, RVASKRAS, GEM231, and INX3001,and antimetabolites such as enocitabine, carmofur, tegafur, pentostatin,doxifluridine, trimetrexate, fludarabine, capecitabine, galocitabine,cytarabine ocfosfate, fosteabine sodium hydrate, raltitrexed,paltitrexid, emitefur, tiazofurin, decitabine, nolatrexed, pemetrexed,nelzarabine, 2′-deoxy-2′-methylidenecytidine,2′-fluoromethylene-2′-deoxycytidine,N-[5-(2,3-dihydro-benzofuryl)sulfonyl]-N′-(3,4-dichlorophenyl)urea,N6-[4-deoxy-4-[N2-[2(E),4(E)-tetradecadienoyl]glycylamino]-L-glycero-B-L-manno-heptopyranosyl]adenine,aplidine, ecteinascidin, troxacitabine,4-[2-amino-4-oxo-4,6,7,8-tetrahydro-3H-pyrimidino[5,4-b][1,4]thiazin-6-yl-(S)-ethyl]-2,5-thienoyl-L-glutamicacid, aminopterin, 5-fluorouracil, alanosine,11-acetyl-8-(carbamoyloxymethyl)-4-formyl-6-methoxy-14-oxa-1,11-diazatetracyclo(7.4.1.0.0)-tetradeca-2,4,6-trien-9-ylacetic acid ester, swainsonine, lometrexol, dexrazoxane, methioninase,2′-cyano-2′-deoxy-N4-palmitoyl-1-B-D-arabino furanosyl cytosine and3-aminopyridine-2-carboxaldehyde thiosemicarbazone.

Examples of monoclonal antibody targeted therapeutic agents includethose therapeutic agents which have cytotoxic agents or radioisotopesattached to a cancer cell specific or target cell specific monoclonalantibody. Examples include Bexxar.

“HMG-CoA reductase inhibitors” refers to inhibitors of3-hydroxy-3-methylglutaryl-CoA reductase. Examples of HMG-CoA reductaseinhibitors that may be used include but are not limited to lovastatin(MEVACOR®; see U.S. Pat. Nos. 4,231,938, 4,294,926 and 4,319,039),simvastatin (ZOCOR®; see U.S. Pat. Nos. 4,444,784, 4,820,850 and4,916,239), pravastatin (PRAVACHOL®; see U.S. Pat. Nos. 4,346,227,4,537,859, 4,410,629, 5,030,447 and 5,180,589), fluvastatin (LESCOL®;see U.S. Pat. Nos. 5,354,772, 4,911,165, 4,929,437, 5,189,164,5,118,853, 5,290,946 and 5,356,896) and atorvastatin (LIPITOR®; see U.S.Pat. Nos. 5,273,995, 4,681,893, 5,489,691 and 5,342,952). The structuralformulas of these and additional HMG-CoA reductase inhibitors that maybe used in the instant methods are described at page 87 of M. Yalpani,“Cholesterol Lowering Drugs”, Chemistry & Industry, pp. 85-89 (5 Feb.1996) and U.S. Pat. Nos. 4,782,084 and 4,885,314. The term HMG-CoAreductase inhibitor as used herein includes all pharmaceuticallyacceptable lactone and open-acid forms (i.e., where the lactone ring isopened to form the free acid) as well as salt and ester forms ofcompounds which have HMG-CoA reductase inhibitory activity, and thereforthe use of such salts, esters, open-acid and lactone forms is includedwithin the scope of this invention.

“Prenyl-protein transferase inhibitor” refers to a compound whichinhibits any one or any combination of the prenyl-protein transferaseenzymes, including farnesyl-protein transferase (FPTase),geranylgeranyl-protein transferase type I (GGPTase-I), andgeranylgeranyl-protein transferase type-II (GGPTase-II, also called RabGGPTase).

Examples of prenyl-protein transferase inhibitors can be found in thefollowing publications and patents: WO 96/30343, WO 97/18813, WO97/21701, WO 97/23478, WO 97/38665, WO 98/28980, WO 98/29119, WO95/32987, U.S. Pat. No. 5,420,245, U.S. Pat. No. 5,523,430, U.S. Pat.No. 5,532,359, U.S. Pat. No. 5,510,510, U.S. Pat. No. 5,589,485, U.S.Pat. No. 5,602,098, European Patent Publ. 0 618 221, European PatentPubl. 0 675 112, European Patent Publ. 0 604 181, European Patent Publ.0 696 593, WO 94/19357, WO 95/08542, WO 95/11917, WO 95/12612, WO95/12572, WO 95/10514, U.S. Pat. No. 5,661,152, WO 95/10515, WO95/10516, WO 95/24612, WO 95/34535, WO 95/25086, WO 96/05529, WO96/06138, WO 96/06193, WO 96/16443, WO 96/21701, WO 96/21456, WO96/22278, WO 96/24611, WO 96/24612, WO 96/05168, WO 96/05169, WO96/00736, U.S. Pat. No. 5,571,792, WO 96/17861, WO 96/33159, WO96/34850, WO 96/34851, WO 96/30017, WO 96/30018, WO 96/30362, WO96/30363, WO 96/31111, WO 96/31477, WO 96/31478, WO 96/31501, WO97/00252, WO 97/03047, WO 97/03050, WO 97/04785, WO 97/02920, WO97/17070, WO 97/23478, WO 97/26246, WO 97/30053, WO 97/44350, WO98/02436, and U.S. Pat. No. 5,532,359. For an example of the role of aprenyl-protein transferase inhibitor on angiogenesis see European J. ofCancer, Vol. 35, No. 9, pp. 1394-1401 (1999).

“Angiogenesis inhibitors” refers to compounds that inhibit the formationof new blood vessels, regardless of mechanism. Examples of angiogenesisinhibitors include, but are not limited to, tyrosine kinase inhibitors,such as inhibitors of the tyrosine kinase receptors Flt-1 (VEGFR1) andFlk-1/KDR (VEGFR2), inhibitors of epidermal-derived, fibroblast-derived,or platelet derived growth factors, MMP (matrix metalloprotease)inhibitors, integrin blockers, interferon-α, interleukin-12, pentosanpolysulfate, cyclooxygenase inhibitors, including nonsteroidalanti-inflammatories (NSAIDs) like aspirin and ibuprofen as well asselective cyclooxy-genase-2 inhibitors like celecoxib and rofecoxib(PNAS, Vol. 89, p. 7384 (1992); JNCI, Vol. 69, p. 475 (1982); Arch.Opthalmol., Vol. 108, p. 573 (1990); Anat. Rec., Vol. 238, p. 68 (1994);FEBS Letters, Vol. 372, p. 83 (1995); Clin, Orthop. Vol. 313, p. 76(1995); J. Mol. Endocrinol., Vol. 16, p. 107 (1996); Jpn. J. Pharmacol.,Vol. 75, p. 105 (1997); Cancer Res., Vol. 57, p. 1625 (1997); Cell, Vol.93, p. 705 (1998); Intl. J. Mol. Med., Vol. 2, p. 715 (1998); J. Biol.Chem., Vol. 274, p. 9116 (1999)), steroidal anti-inflammatories (such ascorticosteroids, mineralocorticoids, dexamethasone, prednisone,prednisolone, methylpred, betamethasone), carboxyamidotriazole,combretastatin A-4, squalamine, 6-O-chloroacetyl-carbonyl)-fumagillol,thalidomide, angiostatin, troponin-1, angiotensin II antagonists (seeFernandez et al., J. Lab. Clin. Med. 105:141-145 (1985)), and antibodiesto VEGF (see, Nature Biotechnology, Vol. 17, pp. 963-968 (October 1999);Kim et al., Nature, 362, 841-844 (1993); WO 00/44777; and WO 00/61186).

Other therapeutic agents that modulate or inhibit angiogenesis and mayalso be used in combination with the compounds of the instant inventioninclude agents that modulate or inhibit the coagulation and fibrinolysissystems (see review in Clin. Chem. La. Med. 38:679-692 (2000)). Examplesof such agents that modulate or inhibit the coagulation and fibrinolysispathways include, but are not limited to, heparin (see Thromb. Haemost.80:10-23 (1998)), low molecular weight heparins and carboxypeptidase Uinhibitors (also known as inhibitors of active thrombin activatablefibrinolysis inhibitor [TAFIa]) (see Thrombosis Res. 101:329-354(2001)). TAFIa inhibitors have been described in PCT Publication WO03/013,526 and U.S. Ser. No. 60/349,925 (filed Jan. 18, 2002).

“Agents that interfere with cell cycle checkpoints” refer to compoundsthat inhibit protein kinases that transduce cell cycle checkpointsignals, thereby sensitizing the cancer cell to DNA damaging agents.Such agents include inhibitors of ATR, ATM, the Chk1 and Chk2 kinasesand cdk and cdc kinase inhibitors and are specifically exemplified by7-hydroxystaurosporin, flavopiridol, CYC202 (Cyclacel) and BMS-387032.

“Inhibitors of cell proliferation and survival signaling pathway” referto pharmaceutical agents that inhibit cell surface receptors and signaltransduction cascades downstream of those surface receptors. Such agentsinclude inhibitors of inhibitors of EGFR (for example gefitinib anderlotinib), inhibitors of ERB-2 (for example trastuzumab), inhibitors ofIGFR, inhibitors of cytokine receptors, inhibitors of MET, inhibitors ofPI3K (for example LY294002), serine/threonine kinases (including but notlimited to inhibitors of Akt such as described in WO 02/083064, WO02/083139, WO 02/083140 and WO 02/083138), inhibitors of Raf kinase (forexample BAY-43-9006), inhibitors of MEK (for example CI-1040 andPD-098059) and inhibitors of mTOR (for example Wyeth CCI-779). Suchagents include small molecule inhibitor compounds and antibodyantagonists.

“Apoptosis inducing agents” include activators of TNF receptor familymembers (including the TRAIL receptors).

The invention also encompasses combinations with NSAID's which areselective COX-2 inhibitors. For purposes of this specification NSAID'swhich are selective inhibitors of COX-2 are defined as those whichpossess a specificity for inhibiting COX-2 over COX-1 of at least 100fold as measured by the ratio of IC₅₀ for COX-2 over IC₅₀ for COX-1evaluated by cell or microsomal assays. Such compounds include, but arenot limited to those disclosed in U.S. Pat. No. 5,474,995, U.S. Pat. No.5,861,419, U.S. Pat. No. 6,001,843, U.S. Pat. No. 6,020,343, U.S. Pat.No. 5,409,944, U.S. Pat. No. 5,436,265, U.S. Pat. No. 5,536,752, U.S.Pat. No. 5,550,142, U.S. Pat. No. 5,604,260, U.S. Pat. No. 5,698,584,U.S. Pat. No. 5,710,140, WO 94/15932, U.S. Pat. No. 5,344,991, U.S. Pat.No. 5,134,142, U.S. Pat. No. 5,380,738, U.S. Pat. No. 5,393,790, U.S.Pat. No. 5,466,823, U.S. Pat. No. 5,633,272, and U.S. Pat. No.5,932,598, all of which are hereby incorporated by reference.

Inhibitors of COX-2 that are particularly useful in the instant methodof treatment are: 3-phenyl-4-(4-(methylsulfonyl)phenyl)-2-(5H)-furanone;and5-chloro-3-(4-methylsulfonyl)phenyl-2-(2-methyl-5-pyridinyl)pyridine;

or a pharmaceutically acceptable salt thereof.

Compounds that have been described as specific inhibitors of COX-2 andare therefore useful in the present invention include, but are notlimited to: parecoxib, CELEBREX® and BEXTRA® or a pharmaceuticallyacceptable salt thereof.

Other examples of angiogenesis inhibitors include, but are not limitedto, endostatin, ukrain, ranpirnase, IM862,5-methoxy-4-[2-methyl-3-(3-methyl-2-butenyl)oxiranyl]-1-oxaspiro[2,5]oct-6-yl(chloroacetyl)carbamate,acetyldinanaline,5-amino-1-[[3,5-dichloro-4-(4-chlorobenzoyl)phenyl]methyl]-1H-1,2,3-triazole-4-carboxamide,CM101, squalamine, combretastatin, RPI4610, NX31838, sulfatedmannopentaose phosphate,7,7-(carbonyl-bis[imino-N-methyl-4,2-pyrrolocarbonylimino[N-methyl-4,2-pyrrole]-carbonylimino]-bis-(1,3-naphthalenedisulfonate), and 3-[(2,4-dimethylpyrrol-5-yl)methylene]-2-indolinone(SU5416).

As used above, “integrin blockers” refers to compounds which selectivelyantagonize, inhibit or counteract binding of a physiological ligand tothe α_(v)β₃ integrin, to compounds which selectively antagonize, inhibitor counteract binding of a physiological ligand to the αvβ5 integrin, tocompounds which antagonize, inhibit or counteract binding of aphysiological ligand to both the α_(v)β₃ integrin and the α_(v)β₅integrin, and to compounds which antagonize, inhibit or counteract theactivity of the particular integrin(s) expressed on capillaryendothelial cells. The term also refers to antagonists of the α_(v)β₆,α_(v)β₈, α₁β₁, α₂β₁, α₅β₁, α₆β₁ and α₆β₄ integrins. The term also refersto antagonists of any combination of α_(v)β₃, α_(v)β₅, α_(v)β₆, α_(v)β₈,α₁β₁, α₂β₁, α₅β₁, α₆β₁ and α₆β₄ integrins.

Some specific examples of tyrosine kinase inhibitors includeN-(trifluoromethylphenyl)-5-methylisoxazol-4-carboxamide,3-[(2,4-dimethylpyrrol-5-yl)methylidenyl)indolin-2-one,17-(allylamino)-17-demethoxygeldanamycin,4-(3-chloro-4-fluorophenylamino)-7-methoxy-6-[3-(4-morpholinyl)propoxyl]quinazoline,N-(3-ethynylphenyl)-6,7-bis(2-methoxyethoxy)-4-quinazolinamine,BIBX1382,2,3,9,10,11,12-hexahydro-10-(hydroxymethyl)-10-hydroxy-9-methyl-9,12-epoxy-1H-diindolo[1,2,3-fg:3′,2′,1′-kl]pyrrolo[3,4-i][1,6]benzodiazocin-1-one,SH268, genistein, STI571, CEP2563,4-(3-chlorophenylamino)-5,6-dimethyl-7H-pyrrolo[2,3-d]pyrimidinemethanesulfonate, 4-(3-bromo-4-hydroxyphenyl)amino-6,7-dimethoxyquinazoline,4-(4′-hydroxyphenyl)amino-6,7-dimethoxyquinazoline, SU6668, STI571A,N-4-chlorophenyl-4-(4-pyridylmethyl)-1-phthalazinamine, and EMD121974.

Combinations with compounds other than anti-cancer compounds are alsoencompassed in the instant methods. For example, combinations of theinstantly claimed compounds with PPAR-γ (i.e., PPAR-gamma) agonists andPPAR-δ (i.e., PPAR-delta) agonists are useful in the treatment ofcertain malignancies. PPAR-γ and PPAR-δ are the nuclear peroxisomeproliferator-activated receptors γ and δ. The expression of PPAR-γ onendothelial cells and its involvement in angiogenesis has been reportedin the literature (see J. Cardiovasc. Pharmacol. 1998; 31:909-913; J.Biol. Chem. 1999; 274:9116-9121; Invest. Ophthalmol Vis. Sci. 2000;41:2309-2317). More recently, PPAR-γ agonists have been shown to inhibitthe angiogenic response to VEGF in vitro; both troglitazone androsiglitazone maleate inhibit the development of retinalneovascularization in mice. (Arch. Ophthamol. 2001; 119:709-717).Examples of PPAR-γ agonists and PPAR-γ/α agonists include, but are notlimited to, thiazolidinediones (such as DRF2725, CS-011, troglitazone,rosiglitazone, and pioglitazone), fenofibrate, gemfibrozil, clofibrate,GW2570, SB219994, AR-H039242, JTT-501, MCC-555, GW2331, GW409544,NN2344, KRP297, NP0110, DRF4158, NN622, GI262570, PNU182716, DRF552926,2-[(5,7-dipropyl-3-trifluoromethyl-1,2-benzisoxazol-6-yl)oxy]-2-methylpropionicacid (disclosed in U.S. Ser. No. 09/782,856), and2(R)-7-(3-(2-chloro-4-(4-fluorophenoxy)phenoxy)propoxy)-2-ethylchromane-2-carboxylicacid (disclosed in U.S. Ser. Nos. 60/235,708 and 60/244,697).

Another embodiment of the instant invention is the use of the presentlydisclosed compounds in combination with gene therapy for the treatmentof cancer. For an overview of genetic strategies to treating cancer seeHall et al (Am J Hum Genet. 61:785-789, 1997) and Kufe et al (CancerMedicine, 5th Ed, pp 876-889, BC Decker, Hamilton 2000). Gene therapycan be used to deliver any tumor suppressing gene. Examples of suchgenes include, but are not limited to, p53, which can be delivered viarecombinant virus-mediated gene transfer (see U.S. Pat. No. 6,069,134,for example), a uPA/uPAR antagonist (“Adenovirus-Mediated Delivery of auPA/uPAR Antagonist Suppresses Angiogenesis-Dependent Tumor Growth andDissemination in Mice,” Gene Therapy, August 1998; 5(8): 1105-13), andinterferon gamma (J Immunol 2000; 164:217-222).

A compound of the instant invention may also be useful for treating orpreventing cancer, including bone cancer, in combination withbisphosphonates (understood to include bisphosphonates, diphosphonates,bisphosphonic acids and diphosphonic acids). Examples of bisphosphonatesinclude but are not limited to: etidronate (Didronel), pamidronate(Aredia), alendronate (Fosamax), risedronate (Actonel), zoledronate(Zometa), ibandronate (Boniva), incadronate or cimadronate, clodronate,EB-1053, minodronate, neridronate, piridronate and tiludronate includingany and all pharmaceutically acceptable salts, derivatives, hydrates andmixtures thereof.

The compounds of the instant invention may also be administered incombination with an inhibitor of inherent multidrug resistance (MDR), inparticular MDR associated with high levels of expression of transporterproteins. Such MDR inhibitors include inhibitors of p-glycoprotein(P-gp), such as LY335979, XR9576, OC144-093, R¹⁰¹⁹²², VX853 and PSC833(valspodar).

A compound of the present invention may be employed in conjunction withanti-emetic agents to treat nausea or emesis, including acute, delayed,late-phase, and anticipatory emesis, which may result from the use of acompound of the present invention, alone or with radiation therapy. Forthe prevention or treatment of emesis, a compound of the presentinvention may be used in conjunction with other anti-emetic agents,especially neurokinin-1 receptor antagonists, 5HT3 receptor antagonists,such as ondansetron, granisetron, tropisetron, and zatisetron, GABABreceptor agonists, such as baclofen, a corticosteroid such as Decadron(dexamethasone), Kenalog, Aristocort, Nasalide, Preferid, Benecorten orothers such as disclosed in U.S. Pat. Nos. 2,789,118, 2,990,401,3,048,581, 3,126,375, 3,929,768, 3,996,359, 3,928,326 and 3,749,712, anantidopaminergic, such as the phenothiazines (for exampleprochlorperazine, fluphenazine, thioridazine and mesoridazine),metoclopramide or dronabinol. In an embodiment, an anti-emesis agentselected from a neurokinin-1 receptor antagonist, a 5HT3 receptorantagonist and a corticosteroid is administered as an adjuvant for thetreatment or prevention of emesis that may result upon administration ofthe instant compounds.

Neurokinin-1 receptor antagonists of use in conjunction with thecompounds of the present invention are fully described, for example, inU.S. Pat. Nos. 5,162,339, 5,232,929, 5,242,930, 5,373,003, 5,387,595,5,459,270, 5,494,926, 5,496,833, 5,637,699, 5,719,147; European PatentPublication Nos. EP 0 360 390, 0 394 989, 0 428 434, 0 429 366, 0 430771, 0 436 334, 0 443 132, 0 482 539, 0 498 069, 0 499 313, 0 512 901, 0512 902, 0 514 273, 0 514 274, 0 514 275, 0 514 276, 0 515 681, 0 517589, 0 520 555, 0 522 808, 0 528 495, 0 532 456, 0 533 280, 0 536 817, 0545 478, 0 558 156, 0 577 394, 0 585 913, 0 590 152, 0 599 538, 0 610793, 0 634 402, 0 686 629, 0 693 489, 0 694 535, 0 699 655, 0 699 674, 0707 006, 0 708 101, 0 709 375, 0 709 376, 0 714 891, 0 723 959, 0 733632 and 0 776 893; PCT International Patent Publication Nos. WO90/05525, 90/05729, 91/09844, 91/18899, 92/01688, 92/06079, 92/12151,92/15585, 92/17449, 92/20661, 92/20676, 92/21677, 92/22569, 93/00330,93/00331, 93/01159, 93/01165, 93/01169, 93/01170, 93/06099, 93/09116,93/10073, 93/14084, 93/14113, 93/18023, 93/19064, 93/21155, 93/21181,93/23380, 93/24465, 94/00440, 94/01402, 94/02461, 94/02595, 94/03429,94/03445, 94/04494, 94/04496, 94/05625, 94/07843, 94/08997, 94/10165,94/10167, 94/10168, 94/10170, 94/11368, 94/13639, 94/13663, 94/14767,94/15903, 94/19320, 94/19323, 94/20500, 94/26735, 94/26740, 94/29309,95/02595, 95/04040, 95/04042, 95/06645, 95/07886, 95/07908, 95/08549,95/11880, 95/14017, 95/15311, 95/16679, 95/17382, 95/18124, 95/18129,95/19344, 95/20575, 95/21819, 95/22525, 95/23798, 95/26338, 95/28418,95/30674, 95/30687, 95/33744, 96/05181, 96/05193, 96/05203, 96/06094,96/07649, 96/10562, 96/16939, 96/18643, 96/20197, 96/21661, 96/29304,96/29317, 96/29326, 96/29328, 96/31214, 96/32385, 96/37489, 97/01553,97/01554, 97/03066, 97/08144, 97/14671, 97/17362, 97/18206, 97/19084,97/19942 and 97/21702; and in British Patent Publication Nos. 2 266 529,2 268 931, 2 269 170, 2 269 590, 2 271 774, 2 292 144, 2 293 168, 2 293169, and 2 302 689. The preparation of such compounds is fully describedin the aforementioned patents and publications, which are incorporatedherein by reference.

In an embodiment, the neurokinin-1 receptor antagonist for use inconjunction with the compounds of the present invention is selectedfrom:2-(R)-(1-(R)-(3,5-bis(trifluoromethyl)phenyl)ethoxy)-3-(S)-(4-fluorophenyl)-4-(3-(5-oxo-1H,4H-1,2,4-triazolo)methyl)morpholine,or a pharmaceutically acceptable salt thereof, which is described inU.S. Pat. No. 5,719,147.

A compound of the instant invention may also be administered with anagent useful in the treatment of anemia. Such an anemia treatment agentis, for example, a continuous eythropoiesis receptor activator (such asepoetin alfa).

A compound of the instant invention may also be administered with anagent useful in the treatment of neutropenia. Such a neutropeniatreatment agent is, for example, a hematopoietic growth factor whichregulates the production and function of neutrophils such as a humangranulocyte colony stimulating factor, (G-CSF). Examples of a G-CSFinclude filgrastim.

A compound of the instant invention may also be administered with animmunologic-enhancing drug, such as levamisole, isoprinosine andZadaxin.

Thus, the scope of the instant invention encompasses the use of theinstantly claimed compounds in combination with a second compoundselected from: an estrogen receptor modulator, an androgen receptormodulator, retinoid receptor modulator, a cytotoxic/cytostatic agent, anantiproliferative agent, a prenyl-protein transferase inhibitor, anHMG-CoA reductase inhibitor, an HIV protease inhibitor, a reversetranscriptase inhibitor, an angiogenesis inhibitor, a PPAR-γ agonist, aPPAR-δ agonist, a bisphosphonate, an inhibitor of inherent multidrugresistance, an anti-emetic agent, an agent useful in the treatment ofanemia, an agent useful in the treatment of neutropenia, animmunologic-enhancing drug, an inhibitor of cell proliferation andsurvival signaling, an agent that interfers with a cell cyclecheckpoint, and an apoptosis inducing agent.

The term “administration” and variants thereof (e.g., “administering” acompound) in reference to a compound of the invention means introducingthe compound or a prodrug of the compound into the system of the animalin need of treatment. When a compound of the invention or prodrugthereof is provided in combination with one or more other active agents(e.g., a cytotoxic agent, etc.), “administration” and its variants areeach understood to include concurrent and sequential introduction of thecompound or prodrug thereof and other agents.

As used herein, the term “composition” is intended to encompass aproduct comprising the specified ingredients in the specified amounts,as well as any product which results, directly or indirectly, fromcombination of the specified ingredients in the specified amounts.

The term “therapeutically effective amount” as used herein means thatamount of active compound or pharmaceutical agent that elicits thebiological or medicinal response in a tissue, system, animal or humanthat is being sought by a researcher, veterinarian, medical doctor orother clinician.

The term “treating cancer” or “treatment of cancer” refers toadministration to a mammal afflicted with a cancerous condition andrefers to an effect that alleviates the cancerous condition by killingthe cancerous cells, but also to an effect that results in theinhibition of growth and/or metastasis of the cancer.

In an embodiment, the angiogenesis inhibitor to be used as the secondcompound is selected from a tyrosine kinase inhibitor, an inhibitor ofepidermal-derived growth factor, an inhibitor of fibroblast-derivedgrowth factor, an inhibitor of platelet derived growth factor, an MMP(matrix metalloprotease) inhibitor, an integrin blocker, interferon-α,interleukin-12, pentosan polysulfate, a cyclooxygenase inhibitor,carboxyamidotriazole, combretastatin A-4, squalamine,6-O-chloroacetyl-carbonyl)-fumagillol, thalidomide, angiostatin,troponin-1, or an antibody to VEGF. In an embodiment, the estrogenreceptor modulator is tamoxifen or raloxifene.

Also included in the scope of the claims is a method of treating cancerthat comprises administering a therapeutically effective amount of acompound of Formula I in combination with radiation therapy and/or incombination with a compound selected from: an estrogen receptormodulator, an androgen receptor modulator, retinoid receptor modulator,a cytotoxic/cytostatic agent, an antiproliferative agent, aprenyl-protein transferase inhibitor, an HMG-CoA reductase inhibitor, anHIV protease inhibitor, a reverse transcriptase inhibitor, anangiogenesis inhibitor, a PPAR-γ agonist, a PPAR-δ agonist, abisphosphonate, an inhibitor of inherent multidrug resistance, ananti-emetic agent, an agent useful in the treatment of anemia, an agentuseful in the treatment of neutropenia, an immunologic-enhancing drug,an inhibitor of cell proliferation and survival signaling, an agent thatinterfers with a cell cycle checkpoint, and an apoptosis inducing agent.

And yet another embodiment of the invention is a method of treatingcancer that comprises administering a therapeutically effective amountof a compound of Formula I in combination with paclitaxel ortrastuzumab.

The invention further encompasses a method of treating or preventingcancer that comprises administering a therapeutically effective amountof a compound of Formula I in combination with a COX-2 inhibitor.

The instant invention also includes a pharmaceutical composition usefulfor treating or preventing cancer that comprises a therapeuticallyeffective amount of a compound of Formula I and a compound selectedfrom: an estrogen receptor modulator, an androgen receptor modulator, aretinoid receptor modulator, a cytotoxic/cytostatic agent, anantiproliferative agent, a prenyl-protein transferase inhibitor, anHMG-CoA reductase inhibitor, an HIV protease inhibitor, a reversetranscriptase inhibitor, an angiogenesis inhibitor, a PPAR-γ agonist, aPPAR-δ agonist; an inhibitor of cell proliferation and survivalsignaling, an agent that interfers with a cell cycle checkpoint, abisphosphonate and an apoptosis inducing agent.

These and other aspects of the invention will be apparent from theteachings contained herein.

Assays

The compounds of the instant invention described in the Examples weretested by the assays described below and were found to have kinesininhibitory activity. Other assays are known in the literature and couldbe readily performed by those of skill in the art (see, for example, PCTPublication WO 01/30768, May 3, 2001, pages 18-22).

I. Kinesin ATPase In Vitro Assay

Cloning and Expression of Human Poly-Histidine Tagged KSP Motor Domain(KSP(367H))

Plasmids for the expression of the human KSP motor domain construct werecloned by PCR using a pBluescript full length human KSP construct(Blangy et al., Cell, vol. 83, pp 1159-1169, 1995) as a template. TheN-terminal primer 5′-GCAACGATTAATATGGCGTCGCAGCCAAATTCGTCTGCGAAG(SEQ.ID.NO.: 1) and the C-terminal primer 5′-GCAACGCTCGAGTCAGTGATGATGGTGGTGATGCTGATTCACTTCAGGCTTATTCAATAT (SEQ.ID.NO.: 2) were used toamplify the motor domain and the neck linker region. The PCR productswere digested with AseI and XhoI, ligated into the NdeI/XhoI digestionproduct of pRSETa (Invitrogen) and transformed into E. coli BL21 (DE3).

Cells were grown at 37° C. to an OD₆₀₀ of 0.5. After cooling the cultureto room temperature expression of KSP was induced with 100 μM IPTG andincubation was continued overnight. Cells were pelleted bycentrifugation and washed once with ice-cold PBS. Pellets wereflash-frozen and stored −80° C.

Protein Purification

Cell pellets were thawed on ice and resuspended in lysis buffer (50 mMK-HEPES, pH 8.0, 250 mM KCl, 0.1% Tween, 10 mM imidazole, 0.5 mM Mg-ATP,1 mM PMSF, 2 mM benzimidine, 1× complete protease inhibitor cocktail(Roche)). Cell suspensions were incubated with 1 mg/ml lysozyme and 5 mMβ-mercaptoethanol on ice for 10 minutes, followed by sonication (3×30sec). All subsequent procedures were performed at 4° C. Lysates werecentrifuged at 40,000×g for 40 minutes. Supernatants were diluted andloaded onto an SP Sepharose column (Pharmacia, 5 ml cartridge) in bufferA (50 mM K-HEPES, pH 6.8, 1 mM MgCl₂, 1 mM EGTA, 10 μM Mg-ATP, 1 mM DTT)and eluted with a 0 to 750 mM KCl gradient in buffer A. Fractionscontaining KSP were pooled and incubated with Ni-NTA resin (Qiagen) forone hour. The resin was washed three times with buffer B (Lysis bufferminus PMSF and protease inhibitor cocktail), followed by three 15-minuteincubations and washes with buffer B. Finally, the resin was incubatedand washed for 15 minutes three times with buffer C (same as buffer Bexcept for pH 6.0) and poured into a column. KSP was eluted with elutionbuffer (identical to buffer B except for 150 mM KCl and 250 mMimidazole). KSP-containing fractions were pooled, made 10% in sucrose,and stored at −80° C.

Microtubules are prepared from tubulin isolated from bovine brain.Purified tubulin (>97% MAP-free) at 1 mg/ml is polymerized at 37° C. inthe presence of 10 μM paclitaxel, 1 mM DTT, 1 mM GTP in BRB80 buffer (80mM K-PIPES, 1 mM EGTA, 1 mM MgCl₂ at pH 6.8). The resulting microtubulesare separated from non-polymerized tubulin by ultracentrifugation andremoval of the supernatant. The pellet, containing the microtubules, isgently resuspended in 10 μM paclitaxel, 1 mM DTT, 50 μg/ml ampicillin,and 5 μg/ml chloramphenicol in BRB80.

The kinesin motor domain is incubated with microtubules, 1 mM ATP (1:1MgCl₂: Na-ATP), and compound at 23° C. in buffer containing 80 mMK-HEPES (pH 7.0), 1 mM EGTA, 1 mM DTT, 1 mM MgCl₂, and 50 mM KCl. Thereaction is terminated by a 2-10 fold dilution with a final buffercomposition of 80 mM HEPES and 50 mM EDTA (or, alternately, with a 1:1addition of reaction volume to stop buffer (1.8M KCl and 50 mM EDTA)).Free phosphate from the ATP hydrolysis reaction is measured via aquinaldine red/ammonium molybdate assay by adding a 1.5 times volume ofquench C (e.g., to a mixture of 40 μl reaction volume+40 μl stop bufferis then added 120 μl quench C). Quench A contains 0.1 mg/ml quinaldinered and 0.14% polyvinyl alcohol; quench B contains 12.3 mM ammoniummolybdate tetrahydrate in 1.15 M sulfuric acid. Quench C is a 2:1 ratioof quench A:quench B The reaction is incubated for 5-10 minutes at 23°C., and the absorbance of the phospho-molybdate complex is measured at540 nm.

The compounds 1-5, 1-7, 1-9, 1-11 and 1-13 in the Examples were testedin the above assay and found to have an IC₅₀>50 ρM.

II. Cell Proliferation Assay

Cells are plated in 96-well tissue culture dishes at densities thatallow for logarithmic growth over the course of 24, 48, and 72 hours andallowed to adhere overnight. The following day, compounds are added in a10-point, one-half log titration to all plates. Each titration series isperformed in triplicate, and a constant DMSO concentration of 0.1% ismaintained throughout the assay. Controls of 0.1% DMSO alone are alsoincluded. Each compound dilution series is made in media without serum.The final concentration of serum in the assay is 5% in a 200 μL volumeof media. Twenty microliters of Alamar blue staining reagent is added toeach sample and control well on the titration plate at 24, 48, or 72hours following the addition of drug and returned to incubation at 37°C. Alamar blue fluorescence is analyzed 6-12 hours later on a CytoFluorII plate reader using 530-560 nanometer wavelength excitation, 590nanometer emission.

A cytotoxic EC₅₀ is derived by plotting compound concentration on thex-axis and average percent inhibition of cell growth for each titrationpoint on the y-axis. Growth of cells in control wells that have beentreated with vehicle alone is defined as 100% growth for the assay, andthe growth of cells treated with compounds is compared to this value.Proprietary in-house software is used to calculate percent cytotoxicityvalues and inflection points using logistic 4-parameter curve fitting.Percent cytotoxicity is defined as:%cytotoxicity:(Fluorescence_(control))−(Flourescence_(sample))×100×(Fluorescence_(control))⁻¹The inflection point is reported as the cytotoxic EC₅₀.III. Evaluation of Mitotic Arrest and Apoptosis by FACS

FACS analysis is used to evaluate the ability of a compound to arrestcells in mitosis and to induce apoptosis by measuring DNA content in atreated population of cells. Cells are seeded at a density of 1.4×10⁶cells per 6 cm² tissue culture dish and allowed to adhere overnight.Cells are then treated with vehicle (0.1% DMSO) or a titration series ofcompound for 8-16 hours. Following treatment, cells are harvested bytrypsinization at the indicated times and pelleted by centrifugation.Cell pellets are rinsed in PBS and fixed in 70% ethanol and stored at 4°C. overnight or longer.

For FACS analysis, at least 500,000 fixed cells are pelleted and the 70%ethanol is removed by aspiration. Cells are then incubated for 30 min at4° C. with RNase A (50 Kunitz units/ml) and propidium iodide (50 μg/ml),and analyzed using a Becton Dickinson FACSCaliber. Data (from 10,000cells) is analyzed using the Modfit cell cycle analysis modelingsoftware (Verity Inc.).

An EC₅₀ for mitotic arrest is derived by plotting compound concentrationon the x-axis and percentage of cells in the G2/M phase of the cellcycle for each titration point (as measured by propidium iodidefluorescence) on the y-axis. Data analysis is performed using theSigmaPlot program to calculate an inflection point using logistic4-parameter curve fitting. The inflection point is reported as the EC₅₀for mitotic arrest. A similar method is used to determine the compoundEC₅₀ for apoptosis. Here, the percentage of apoptotic cells at eachtitration point (as determined by propidium iodide fluorescence) isplotted on the y-axis, and a similar analysis is carried out asdescribed above.

IV. Immunofluorescence Microscopy to Detect Monopolar Spindles

Methods for immunofluorescence staining of DNA, tubulin, and pericentrinare essentially as described in Kapoor et al. (2000) J. Cell Biol. 150:975-988. For cell culture studies, cells are plated on tissue culturetreated glass chamber slides and allowed to adhere overnight. Cells arethen incubated with the compound of interest for 4 to 16 hours. Afterincubation is complete, media and drug are aspirated and the chamber andgasket are removed from the glass slide. Cells are then permeabilized,fixed, washed, and blocked for nonspecific antibody binding according tothe referenced protocol. Paraffin-embedded tumor sections aredeparaffinized with xylene and rehydrated through an ethanol seriesprior to blocking. Slides are incubated in primary antibodies (mousemonoclonal anti-α-tubulin antibody, clone DM1A from Sigma diluted 1:500;rabbit polyclonal anti-pericentrin antibody from Covance, diluted1:2000) overnight at 4° C. After washing, slides are incubated withconjugated secondary antibodies (FITC-conjugated donkey anti-mouse IgGfor tubulin; Texas red-conjugated donkey anti-rabbit IgG forpericentrin) diluted to 15 μg/ml for one hour at room temperature.Slides are then washed and counterstained with Hoechst 33342 tovisualize DNA. Immunostained samples are imaged with a 100× oilimmersion objective on a Nikon epifluorescence microscope usingMetamorph deconvolution and imaging software.

EXAMPLES

Examples provided are intended to assist in a further understanding ofthe invention. Particular materials employed, species and conditions areintended to be illustrative of the invention and not limiting of thereasonable scope thereof.

Step 1: Synthesis of 1-1

To a suspension of 24.9 g (255 mmol) N,O-dimethylhydroxylaminehydrochloride in 1.5 L of CH₂Cl₂ at 0° C. was added 71.1 mL (510 mmol)triethylamine, followed by dropwise addition of 25.0 g (141.6 mmol)2,5-difluorobenzoyl chloride in 100 mL CH₂Cl₂. After stirring for 1 h,the reaction was washed with water, three times with 1M HCl, brine,dried over Na₂SO₄, and concentrated to provide 1-1 as a colorless oil.Data for 1-1: ¹HNMR (500 MHz, CDCl₃) δ 7.2-7.0 (m, 3H), 3.6 (bs, 3H),3.4 (s, 3H) ppm.

Step 2: Synthesis of1-(2,5-difluorophenyl)-6-(tetrahydro-2H-pyran-2-yloxy)hex-2-yn-1-one(1-3)

To a solution of 15.0 g (89.2 mmol) of THP-alkyne 1-2 (prepared asdescribed in Tetrahedron, 2001, 57, 2597-2608) in 750 mL of THF at −78°C. was added 35.7 mL (89.2 mmol) of 2.5M nBuLi in hexanes. Afterstirring for 1 h at that temperature, a solution of 17.9 g (89.2 mmol)of 1-1 in 35 mL of THF was added via syringe. The solution was stirredovernight while gradually warming to room temperature. The reaction wasquenched with saturated aqueous NH₄Cl and extracted with EtOAc. Theorganic extract was washed with brine, dried over Na₂SO₄, concentratedby rotary evaporation, and purified by column chromatography withEtOAc/hexanes to provide 1-3 as a colorless oil. Data for 1-3: ¹HNMR(500 MHz, CDCl₃) δ 7.7 (m, 1H), 7.3 (m, 1H), 7.15 (m, 1H), 4.6 (m, 1H),3.9 (m, 2H), 3.55 (m, 2H), 2.65 (m, 2H), 1.95 (m, 2H), 1.85 (m, 1H),1.75 (m, 1H), 1.6-1.5 (m, 4H) ppm.

Step 3: Synthesis of1-(2,5-difluorophenyl)-3-phenyl-6-(tetrahydro-2H-pyran-2-yloxy)hex-2-en-1-one(1-4)

To a suspension of 20.2 g (98.1 mmol) of copper(I) bromidedimethylsulfide complex in 75 mL of THF at −78° C. was added 98.1 mL(196 mmol) of a 2M solution of PhLi in dibutylether. After stirring for1.5 h, 25.2 g (81.7 mmol) of alkyne 1-3 in 200 mL of THF was added viacannula, and the reaction was stirred for 3 h at −78° C. The reactionwas quenched with saturated aqueous NH₄Cl and extracted twice withEtOAc. The organic extracts were combined, washed with brine, dried overNa₂SO₄, concentrated by rotary evaporation, and purified by columnchromatography with EtOAc/hexanes to provide 1-4 as a yellow oil,mixture of (E) and (Z) isomers. Data for 1-4: HRMS (ES) calc'd M+Na forC₂₃H₂₄F₂O₃: 409.1586. Found: 409.1586.

Step 4: Synthesis of3-[1-acetyl-3-(2,5-difluorophenyl)-5-phenyl-4,5-dihydro-1H-pyrazol-5-yl]propan-1-ol(1-5)

To a solution of 16.0 g (41.4 mmol) of 1-4 in 150 mL of pyridine wasadded 3.0 mL (62.1 mmol) of hydrazine hydrate, and the resulting mixturewas heated at 90° C. for 45 min. After cooling to room temperature, thereaction was placed in an ice bath and 14.7 mL (207 mmol) of acetylchloride was added dropwise. The bath was removed and the reaction wasstirred overnight at room temperature before being dumped into aseparatory funnel containing EtOAc and brine. The layers were separated,the organic was washed twice with 1M HCl, once with brine, dried overNa₂SO₄, and concentrated by rotary evaporation. The residue was twiceresuspended in toluene and concentrated to azeotrope off any remainingpyridine. The residue was dissolved in 200 mL of MeOH and 8.0 g (42mmol) of p-toluenesulfonic acid monohydrate was added. After stirringfor 2 h at room temperature, most of the solvent was removed by rotaryevaporation and the residue partitioned between saturated aqueous NaHCO₃and EtOAc. After separating the layers, the organic was washed againwith bicarbonate, then brine, dried over Na₂SO₄, and concentrated byrotary evaporation. The residue was triturated with Et₂O and the solidmaterial was collected by filtration to provide racemic 1-5 as a whitesolid. Data for racemic 1-5: ¹HNMR (500 MHz, CDCl₃) δ 7.7 (m, 1H),7.4-7.0 (m, 7H), 3.8-3.65 (m, 2H), 3.6 (m, 1H), 3.5 (m, 1H), 2.9 (m,1H), 2.4 (s, 3H), 2.3 (m, 1H), 1.7-1.5 (m, 2H) ppm. HRMS (ES) calc'd M+Hfor C₂₀H₂₀F₂N₂O₃: 359.1566. Found: 359.1557

Step 5: Chiral Resolution of3-[(5S)-1-acetyl-3-(2,5-difluorophenyl)-5-phenyl-4,5-dihydro-1H-pyrazol-5-yl]propan-1-ol1-5

The enantiomers were resolved by preparative HPLC with a 10 cm ChiralpakAD column with an eluant of 92% hexanes (with 0.1% diethylamine), 4%MeOH, and 4% EtOH at 150 mL/min. The first peak to elute was theinactive (R) isomer, the second to elute was the active (S) isomer.

Step 6: Synthesis of3-[(S)-1-acetyl-3-(2,5-difluorophenyl)-5-phenyl-4,5-dihydro-1H-pyrazol-5-yl]propyldihydrogen phosphate 1-6

To a solution of 250 mg (0.70 mmol) of (S)-1-5 in 10 mL of CH₂Cl₂ wasadded 292 μL (2.10 mmol) triethylamine, 151 μL (1.40 mmol) dimethylchlorophosphonate, and 24 mg (0.07 mmol) titanium (IV) tert-butoxide.After stirring overnight at room temperature, the reaction waspartitioned between EtOAc and saturated aqueous NaHCO₃. The layers wereseparated, the organic layer was washed with water, brine, dried overMgSO₄ and concentrated by rotary evaporation. The residue was dissolvedin 0.5 mL of dimethylsulfide and 1 mL methanesulfonic acid and themixture was allowed to stir overnight at room temperature. The reactionwas then diluted with a small amount of CH₃CN and purified bypreparative HPLC on a Delta Pak C₁₈ column with CH₃CN/H₂O (plus 0.1%TFA). The fractions containing product were lyophilized to provide 1-6as a fluffy white solid. Data for 1-6: ¹HNMR (500 MHz, d₆-DMSO) δ 7.65(m, 1H), 7.4 (m, 4H), 7.2 (m, 3H), 3.9 (m, 2H), 3.7 (m, 1H), 3.4 (m,1H), 2.6 (m, 1H), 2.3 (s, 3H), 2.3 (m, 1H), 1.7 (m, 1H), 1.4 (m, 1H)ppm; HRMS (APCI) calc'd M+H for C₂₀H₂₁F₂N₂O₅P: 439.1229. Found:439.1231.

The following compounds were prepared by simple modifications of theabove procedures. Compounds 1-13 and 1-14 were isolated as racemicmixtures. Unless otherwise indicated, the compounds in the table wereisolated as the free base.

HRMS m/z Cmpd RP R^(7′) n Name (M + H) 1-7 H CH₃ 02-[(5R)-1-acetyl-3-(2-fluoro- HRMS m/z 5-methylphenyl)-5-phenyl- (M + H)341.1521 4,5-dihydro-1H-pyrazol-5- found, 341.1660 yl]ethanol required.1-8 P(O)(OH)₂ CH₃ 0 2-[(5R)-1-acetyl-3-(2-fluoro- HRMS m/z5-methylphenyl)-5-phenyl- (M + H) 421.1325 4,5-dihydro-1H-pyrazol-5-found, 421.1323 yl]ethyl dihydrogen phosphate required 1-9 H CH₃ 12-[(5R)-1-acetyl-3-(2-fluoro- HRMS m/z 5-methylphenyl)-5-phenyl- (M + H)355.1798 4,5-dihydro-1H-pyrazol-5- found, 355.1817 yl]propan-1-olrequired 1-10 P(O)(OH)₂ CH₃ 1 2-[(5R)-1-acetyl-3-(2-fluoro- HRMS m/z5-methylphenyl)-5-phenyl- (M + H) 435.1477 4,5-dihydro-1H-pyrazol-5-found, 435.1480 yl]propyl dihydrogen phosphate required 1-11 H CH₃ 22-[(5R)-1-acetyl-3-(2-fluoro- HRMS m/z 5-methylphenyl)-5-phenyl- (M + H)369.1971 4,5-dihydro-1H-pyrazol-5- found, 369.1973 yl]butan-1-olrequired 1-12 P(O)(OH)₂ CH₃ 2 2-[(5R)-1-acetyl-3-(2-fluoro- HRMS m/z5-methylphenyl)-5-phenyl- (M + H) 449.1630 4,5-dihydro-1H-pyrazol-5-found, 449.1636 yl]butyl dihydrogen phosphate required 1-13 H Br 13-[1-acetyl-3-(5-bromo-2- HRMS m/z fluorophenyl)-5-phenyl-4,5- (M + H)419.0769 dihydro-1H-pyrazol-5- found, 419.0765 yl]propan-1-ol required1-14 P(O)(OH)₂ Br 1 3-[1-acetyl-3-(5-bromo-2- HRMS m/zfluorophenyl)-5-phenyl-4,5- (M + H) 499.0423 dihydro-1H-pyrazol-5-found, 499.0428 yl]propyl dihydrogen phosphate required

1. A compound of Formula I:

or a pharmaceutically acceptable salt or stereoisomer thereof, wherein ais independently 0 or 1; b is independently 0 or 1; m is independently0, 1, or 2; n is 0 to 8; p is 1 to 6; u is 1, 2, 3, 4 or 5; R¹ isselected from: 1) (C═O)C₁-C₁₀ alkyl, 2) (C═O)aryl, 3) (C═O)C₂-C₁₀alkenyl, 4) (C═O)C₂-C₁₀ alkynyl, 5) (C═O)C₃-C₈ cycloalkyl, 6)(C═O)heterocyclyl, 7) (C═O)NR⁹R¹⁰, 8) (C═O)OC₁-C₁₀ alkyl, 15)heteroaryl, 16) (CH₂)_(u)(C═O)C₁-C₁₀ alkyl, 17) (CH₂)_(u)(C═O)NR⁹R¹⁰,18) 3-pyrrolidinonyl, 3-piperidinonyl, 2-cyclopentanonyl,2-cyclohexanonyl, 19) (C═O)(C═O)C₁-C₁₀ alkyl, 20) (C═O)(C═O)NR⁹R¹⁰, 21)(C═O)(C═O)OC₁-C₁₀ alkyl, said alkyl, aryl, alkenyl, alkynyl, cycloalkyl,heteroaryl and heterocyclyl is optionally substituted with one or moresubstituents selected from R⁷; or R² is selected from: 1) C₁-C₁₀ alkyl,2) aryl, 3) C₂-C₁₀ alkenyl, 4) C₂-C₁₀ alkynyl, 5) C₁-C₆ perfluoroalkyl,6) C₁-C₆ aralkyl, 7) C₁-C₆ heteroaralkyl, 8) C₃-C₈ cycloalkyl, and 9)heterocyclyl, said alkyl, aryl, alkenyl, alkynyl, cycloalkyl, aralkyl,heteroaralkyl and heterocyclyl is optionally substituted with one ormore substituents selected from R⁷; R³, R⁴ and R⁵ are independentlyselected from: 1) H, 2) C₁-C₁₀ alkyl, 3) aryl, 4) C₂-C₁₀ alkenyl, 5)C₂-C₁₀ alkynyl, 6) C₁-C₆ perfluoroalkyl, 7) C₁-C₆ aralkyl, 8) C₃-C₈cycloalkyl, and 9) heterocyclyl, said alkyl, aryl, alkenyl, alkynyl,cycloalkyl, aralkyl and heterocyclyl is optionally substituted with oneor more substituents selected from R⁷; or R³ and R⁴ attached to the samecarbon atom are combined to form —(CH₂)_(u)— wherein one of the carbonatoms is optionally replaced by a moiety selected from O, S(O)_(m),—N(R⁹)C(O)—, and —N(COR¹⁰)—; R⁷ is: 1) (CO)_(a)O_(b)C₁-C₁₀ alkyl, 2)(C═O)_(a)O_(b)aryl, 3) C₂-C₁₀ alkenyl, 4) C₂-C₁₀ alkynyl, 5)(CO)_(a)O_(b) heterocyclyl, 6) CO₂H, 7) halo, 8) CN, 9) OH, 10)O_(b)C₁-C₆ perfluoroalkyl, 11) O_(a)(C═O)_(b)NR⁹R¹⁰, 12) S(O)_(m)R^(a),13) S(O)₂NR⁹R¹⁰, 14) oxo, 15) CHO, 16) (N═O)R⁹R¹⁰, or 17)(C═O)_(a)O_(b)C₃-C₈ cycloalkyl, said alkyl, aryl, alkenyl, alkynyl,heterocyclyl, and cycloalkyl optionally substituted with one or moresubstituents selected from R⁸; R⁸ is selected from: 1)(C═O)_(a)O_(b)(C₁-C₁₀)alkyl, 2) O_(b)(C₁-C₃)perfluoroalkyl, 3)(C₀-C₆)alkylene-S(O)_(m)R^(a); 4) oxo, 5) OH, 6) halo, 7) CN, 8)(C═O)_(a)O_(b)(C₂-C₁₀)alkenyl, 9) (C═O)_(a)O_(b)(C₂-C₁₀)alkynyl, 10)(CO)_(a)O_(b)(C₃-C₆)cycloalkyl, 11) (C═O)_(a)O_(b)(C₀-C₆)alkylene-aryl,12) (C═O)_(a)O_(b)(C₀-C₆)alkylene-heterocyclyl, 13)(C═O)_(a)O_(b)(C₀-C₆)alkylene-N(R^(b))₂, 14) C(O)R^(a), 15)(C₀-C₆)alkylene-CO₂R^(a), 16) C(O)H, 17) (C₀-C₆)alkylene-CO₂H, 18)C(O)N(R^(b))₂, 19) S(O)_(m)R^(a), 20) S(O)₂NR⁹R¹⁰, and 21) C(NH)NH₂;said alkyl, alkenyl, alkynyl, cycloalkyl, aryl, and heterocyclyl isoptionally substituted with up to three substituents selected fromR^(b), OH, (C₁-C₆)alkoxy, halogen, CO₂H, CN, O(C═O)C₁-C₆ alkyl, oxo, andN(R^(b))₂; R⁹ and R¹⁰ are independently selected from: 1) H, 2)(C═O)O_(b)C₁-C₁₀ alkyl, 3) (C═O)O_(b)C₃-C₈ cycloalkyl, 4)(C═O)O_(b)aryl, 5) (C═O)O_(b)heterocyclyl, 6) C₁-C₁₀alkyl, 7) aryl, 8)C₂-C₁₀ alkenyl, 9) C₂-C₁₀ alkynyl, 10) heterocyclyl, 11) C₃-C₈cycloalkyl, 12) SO₂R^(a), and 13) (C═O)NR^(b) ₂, said alkyl, cycloalkyl,aryl, heterocylyl, alkenyl, and alkynyl is optionally substituted withone or more substituents selected from R⁸, or R⁹ and R¹⁰ can be takentogether with the nitrogen to which they are attached to form amonocyclic or bicyclic heterocycle with 3-7 members in each ring andoptionally containing, in addition to the nitrogen, one or twoadditional heteroatoms selected from N, O and S, said monocyclic orbicyclic heterocycle optionally substituted with one or moresubstituents selected from R⁸; R^(a) is (C₁-C₆)alkyl, (C₃-C₆)cycloalkyl,aryl, benzyl or heterocyclyl; R^(b) is H, (C₁-C₆)alkyl, aryl,heterocyclyl, (C₃-C₆)cycloalkyl, (C═O)OC₁-C₆ alkyl, (C═O)C₁-C₆ alkyl orS(O)₂R^(a); and R^(c) is independently selected from: (C₁-C₆)alkoxy,pivaloyloxymethoxy and NR^(b) ₂, or two R^(c)s can be taken togetherwith the phosphorous to which they are attached to form a monocyclicheterocycle with 5-7 members said monocyclic heterocycle optionallysubstituted with one, two or three substituents selected from R^(b);R^(d) is independently selected from H, (C₁-C₆)alkyl, (C₃-C₆)cycloalkyl,aryl, benzyl or heterocyclyl; R^(P) is selected from: PO(OH)₂,PO(R^(c))₂, C(O)CH₂CH₂CH₂OPO(OH)₂, C(O)CH₂CH₂CH₂OPO(R^(c))₂,—CH₂OPO(OH)₂, —CH₂OPO(R^(c))₂, and


2. The compound according to claim 1 of the Formula II:

or a pharmaceutically acceptable salt or stereoisomer thereof, wherein:a is independently 0 or 1; b is independently 0 or 1; m is independently0, 1, or 2; n is 0 to 5; R¹ is selected from: 1) (C═O)C₁-C₁₀ alkyl, 2)(C═O)aryl, 3) (C═O)C₂-C₁₀ alkenyl, 4) (C═O)C₂-C₁₀ alkynyl, 5) (C═O)C₃-C₈cycloalkyl, 6) (C═O)heterocyclyl, 7) (C═O)OC₁-C₁₀ alkyl, 8) (C═O)NR⁹R¹⁰,said alkyl, aryl, alkenyl, alkynyl, cycloalkyl, and heterocyclyl isoptionally substituted with one or more substituents selected from R⁷;or R² is selected from: 1) C₁-C₁₀ alkyl, 2) aryl, 3) C₂-C₁₀ alkenyl, 4)C₂-C₁₀ alkynyl, 5) C₁-C₆ perfluoroalkyl, 6) C₁-C₆ aralkyl, 7) C₃-C₈cycloalkyl, and 8) heterocyclyl, said alkyl, aryl, alkenyl, alkynyl,cycloalkyl, aralkyl and heterocyclyl is optionally substituted with oneor more substituents selected from R⁷; R³, R⁴ and R⁵ are independentlyselected from: 1) H, 2) C₁-C₁₀alkyl, 3) aryl, 4) C₂-C₁₀ alkenyl, 5)C₂-C₁₀ alkynyl, 6) C₁-C₆ perfluoroalkyl, 7) C₁-C₆ aralkyl, 8) C₃-C₈cycloalkyl, and 9) heterocyclyl, said alkyl, aryl, alkenyl, alkynyl,cycloalkyl, aralkyl and heterocyclyl is optionally substituted with oneor more substituents selected from R⁷; R⁷ is: 1) (C═O)_(a)O_(b)C₁-C₁₀alkyl, 2) (C═O)_(a)O_(b)aryl, 3) C₂-C₁₀ alkenyl, 4) C₂-C₁₀ alkynyl, 5)(C═O)_(a)O_(b) heterocyclyl, 6) CO₂H, 7) halo, 8) CN, 9) OH, 10)O_(b)C₁-C₆ perfluoroalkyl, 11) O_(a)(C═O)_(b)NR⁹R¹⁰, 12) S(O)_(m)R^(a),13) S(O)₂NR⁹R¹⁰, 14) oxo, 15) CHO, 16) (N═O)R⁹R¹⁰, or 17)(C═O)_(a)O_(b)C₃-C₈ cycloalkyl, said alkyl, aryl, alkenyl, alkynyl,heterocyclyl, and cycloalkyl optionally substituted with one, two orthree substituents selected from R⁸; R⁸ is selected from: 1)(C═O)_(a)O_(b)(C₁-C₁₀)alkyl, 2) O_(b)(C₁-C₃)perfluoroalkyl, 3) oxo, 4)OH, 5) halo, 6) CN, 7) (C₂-C₁₀)alkenyl, 8) (C₂-C₁₀)alkynyl, 9)(CO)_(a)O_(b)(C₃-C₆)cycloalkyl, 10) (C═O)_(a)O_(b)(C₀-C₆)alkylene-aryl,11) (C═O)_(a)O_(b)(C₀-C₆)alkylene-heterocyclyl, 12)(CO)_(a)O_(b)(C₀-C₆)alkylene-N(R^(b))₂, 13) C(O)R^(a), 14)(C₀-C₆)alkylene-CO₂R^(a), 15) C(O)H, 16) (C₀-C₆)alkylene-CO₂H, and 17)C(O)N(R^(b))₂, 18) S(O)_(m)R^(a), and 19) S(O)₂NR⁹R¹⁰; said alkyl,alkenyl, alkynyl, cycloalkyl, aryl, and heterocyclyl is optionallysubstituted with up to three substituents selected from R^(b), OH,(C₁-C₆)alkoxy, halogen, CO₂H, CN, O(C═O)C₁-C₆ alkyl, oxo, and N(R^(b))₂;R⁹ and R¹⁰ are independently selected from: 1) H, 2) (C═O)O_(b)C₁-C₁₀alkyl, 3) (C═O)O_(b)C₃-C₈ cycloalkyl, 4) (C═O)O_(b)aryl, 5)(C═O)O_(b)heterocyclyl, 6) C₁-C₁₀alkyl, 7) aryl, 8) C₂-C₁₀ alkenyl, 9)C₂-C₁₀ alkynyl, 10) heterocyclyl, 11) C₃-C₈ cycloalkyl, 12) SO₂R^(a),and 13) (C═O)NR^(b) ₂, said alkyl, cycloalkyl, aryl, heterocylyl,alkenyl, and alkynyl is optionally substituted with one, two or threesubstituents selected from R⁸, or R⁹ and R¹⁰ can be taken together withthe nitrogen to which they are attached to form a monocyclic or bicyclicheterocycle with 5-7 members in each ring and optionally containing, inaddition to the nitrogen, one or two additional heteroatoms selectedfrom N, O and S, said monocyclic or bicyclic heterocycle optionallysubstituted with one, two or three substituents selected from R⁸; R^(a)is (C₁-C₆)alkyl, (C₃-C₆)cycloalkyl, aryl, or heterocyclyl; R^(b) is H,(C₁-C₆)alkyl, aryl, heterocyclyl, (C₃-C₆)cycloalkyl, (C═O)OC₁-C₆ alkyl,(C═O)C₁-C₆ alkyl or S(O)₂R^(a); and R^(c) is independently selectedfrom: (C₁-C₆)alkoxy, pivaloyloxymethoxy and NR^(b) ₂, or two R^(c)s canbe taken together with the phosphorous to which they are attached toform a monocyclic heterocycle with 5-7 members said monocyclicheterocycle optionally substituted with one, two or three substituentsselected from R^(b); R^(p) is selected from: PO(OH)₂, —PO(R^(c))₂,C(O)CH₂CH₂CH₂OPO(OH)₂, C(O)CH₂CH₂CH₂OPO(R^(c))₂, and


3. The compound according to claim 2 of Formula III:

or a pharmaceutically acceptable salt or stereoisomer thereof, wherein ais independently 0 or 1; b is independently 0 or 1; m is independently0, 1, or 2; n is 0 to 5; R¹ is selected from: 1) (C═O)C₁-C₁₀ alkyl, 2)(C═O)aryl, 3) (C═O)C₂-C₁₀ alkenyl, 4) (C═O)C₂-C₁₀ alkynyl, 5) (C═O)C₃-C₈cycloalkyl, 6) (C═O)heterocyclyl, 7) (C═O)OC₁-C₁₀ alkyl, 8) (C═O)NR⁹R¹⁰,said alkyl, aryl, alkenyl, alkynyl, cycloalkyl, and heterocyclyl isoptionally substituted with one or more substituents selected from R⁷;or R^(7a) and R^(7c) are independently selected from: 1) H, 2)O_(b)C₁-C₁₀ alkyl, 3) halo, 4) CN, and 5) OH, said alkyl optionallysubstituted with one, two or three substituents selected from R⁸; R^(7b)is halogen; R⁷ is: 1) (C═O)_(a)O_(b)C₁-C₁₀ alkyl, 2) (C═O)_(a)O_(b)aryl,3) C₂-C₁₀ alkenyl, 4) C₂-C₁₀ alkynyl, 5) (C═O)_(a)O_(b) heterocyclyl, 6)CO₂H, 7) halo, 8) CN, 9) OH, 10) O_(b)C₁-C₆ perfluoroalkyl, 11)O_(a)(C═O)_(b)NR⁹R¹⁰, 12) S(O)_(m)R^(a), 13) S(O)₂NR⁹R¹⁰, 14) oxo, 15)CHO, 16) (N═O)R⁹R¹⁰, or 17) (C═O)_(a)O_(b)C₃-C₈ cycloalkyl, said alkyl,aryl, alkenyl, alkynyl, heterocyclyl, and cycloalkyl optionallysubstituted with one or more substituents selected from R⁸; R⁸ isselected from: 1) (C═O)_(a)O_(b)(C₁-C₁₀)alkyl, 2)O_(b)(C₁-C₃)perfluoroalkyl, 3) oxo, 4) OH, 5) halo, 6) CN, 7)(C₂-C₁₀)alkenyl, 8) (C₂-C₁₀)alkynyl, 9) (CO)_(a)O_(b)(C₃-C₆)cycloalkyl,10) (C═O)_(a)O_(b)(C₀-C₆)alkylene-aryl, 11)(C═O)_(a)O_(b)(C₀-C₆)alkylene-heterocyclyl, 12)(C═O)_(a)O_(b)(C₀-C₆)alkylene-N(R^(b))₂, 13) C(O)R^(a), 14)(C₀-C₆)alkylene-CO₂R^(a), 15) C(O)H, 16) (C₀-C₆)alkylene-CO₂H, and 17)C(O)N(R^(b))₂, 18) S(O)_(m)R^(a), and 19) S(O)₂NR⁹R¹⁰; said alkyl,alkenyl, alkynyl, cycloalkyl, aryl, and heterocyclyl is optionallysubstituted with up to three substituents selected from R^(b), OH,(C₁-C₆)alkoxy, halogen, CO₂H, CN, O(C═O)C₁-C₆ alkyl, oxo, and N(R^(b))₂;R⁹ and R¹⁰ are independently selected from: 1) H, 2) (C═O)O_(b)C₁-C₁₀alkyl, 3) (C═O)O_(b)C₃-C₈ cycloalkyl, 4) (C═O)O_(b)aryl, 5)(C═O)O_(b)heterocyclyl, 6) C₁-C₁₀alkyl, 7) aryl, 8) C₂-C₁₀ alkenyl, 9)C₂-C₁₀ alkynyl, 10) heterocyclyl, 11) C₃-C₈ cycloalkyl, 12) SO₂R^(a),and 13) (C═O)NR^(b) ₂, said alkyl, cycloalkyl, aryl, heterocylyl,alkenyl, and alkynyl is optionally substituted with one, two or threesubstituents selected from R⁸, or R⁹ and R¹⁰ can be taken together withthe nitrogen to which they are attached to form a monocyclic or bicyclicheterocycle with 5-7 members in each ring and optionally containing, inaddition to the nitrogen, one or two additional heteroatoms selectedfrom N, O and S, said monocyclic or bicyclic heterocycle optionallysubstituted with one, two or three substituents selected from R⁸; R^(a)is independently selected from: (C₁-C₆)alkyl, (C₃-C₆)cycloalkyl, aryl,and heterocyclyl; R^(b) is independently selected from: H, (C₁-C₆)alkyl,aryl, heterocyclyl, (C₃-C₆)cycloalkyl, (C═O)OC₁-C₆ alkyl, (C═O)C₁-C₆alkyl or S(O)₂R^(a); and R^(p) is selected from: —PO(OH)₂,—C(O)CH₂CH₂CH₂OPO(OH)₂, —CH₂OPO(OH)₂, and


4. The compound according to claim 3 of the Formula III wherein R^(p)is: —PO(OH)₂ and all of the remaining substituents are as described inclaim
 3. 5. A pharmaceutical composition that is comprised of a compoundin accordance with claim 1 and a pharmaceutically acceptable carrier. 6.The compound of claim 1 selected from:3-[(5S)-1-acetyl-3-(2,5-difluorophenyl)-5-phenyl-4,5-dihydro-1H-pyrazol-5-yl]propyldihydrogen phosphate;2-[(5R)-1-acetyl-3-(2-fluoro-5-methylphenyl)-5-phenyl-4,5-dihydro-1H-pyrazol-5-yl]ethyldihydrogen phosphate;2-[(5R)-1-acetyl-3-(2-fluoro-5-methylphenyl)-5-phenyl-4,5-dihydro-1H-pyrazol-5-yl]propyldihydrogen phosphate;2-[(5R)-1-acetyl-3-(2-fluoro-5-methylphenyl)-5-phenyl-4,5-dihydro-1H-pyrazol-5-yl]butyldihydrogen phosphate; and3-[(5S)-1-acetyl-3-(5-bromo-2-fluorophenyl)-5-phenyl-4,5-dihydro-1H-pyrazol-5-yl]propyldihydrogen phosphate; or a pharmaceutically acceptable salt orstereoisomer thereof.